A kind of high-efficiency breeding method of clematis

Abstract

The invention provides an efficient breeding method for anoectochilus roxburghii. The breeding method comprises the following steps: by breaking through a conventional breeding method form of anoectochilus roxburghii, culturing and screening anoectochilus roxburghii chromosomes doubled of golden leaves to obtain a polyploidy plant; selecting at least one target polyploidy plant with the golden leaves in the polyploidy plant; carrying out batched culture according to a cottage method; culturing the plants to multiple first target seedlings; performing a first stage of golden variety optimization on the target polyploidy plants and selecting the target polyploidy plants with the golden yellow blades as the first stage target seedlings; performing a second stage of gold variety optimization on the first stage target seedlings cultured for a period of time and selecting at least one first stage target seedling with the yellowiest leaves as the second stage target seedlings; then, carryingout batched cultivation by means of cottage. Compared with the prior art, the efficient breeding method for anoectochilus roxburghii can be used for efficient and stable batched breeding on the polyploidy anoectochilus roxburghii, so that the yellow property of the anoectochilus roxburghii is improved.

Description

technical field [0001] The invention relates to the field of plant breeding methods, in particular to a high-efficiency breeding method for Aromatica clematis. Background technique [0002] The breeding method of polyploid clematis is known, as Chinese invention patent CN201510749602.1 discloses a breeding method of polyploid clematis species, comprising the following steps: (a), diploid clematis The seeds are cultured to obtain sprouts; (b), soak the sprouts in a 5-15 μmol / L asulphin solution for 10-36 hours; (c), the soaked sprouts continue to be cultivated to obtain clustered sprouts; (d), the clustered shoots are divided into rooting medium and then cultured to obtain tissue cultured plantlets with complete roots, stems and leaves; (e), the tissue cultured plantlets are screened to obtain polyploid plants. In step (a) and step (c), the cultivation is carried out using the first medium; the first medium is: MS medium containing BA 0.8-1.2mg / L, KT0.7-1.2mg / L, NAA0.05-0....

AI Technical Summary

Problems solved by technology

[0003] The steps of this invention are simple, but it is similar to other breeding methods on the market, all can't carry out selective breeding to golden clematis (the golden clematis of golden yellow leaf), more can't strengthen the yellow nature of golden lotus to improve golden golden lotus quality, and fact In terms of medicinal value and market, golden lotus is more likely to be recognized

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