Potato seedling stage freezing tolerance determination method

An identification method and freeze-tolerance technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as insufficient frost damage, uncontrolled freezing time and temperature, and achieve good technical results and accurate and reliable data.

Inactive Publication Date: 2019-03-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

Its disadvantage is that it is easily affected by seasons and ambient temperature. It needs to be in a special season. At the same time, the freezing time and temperature cannot be controlled artificially. Therefore, it is necessary to check the weather forecast in advance to grasp the weather conditions

Method used

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  • Potato seedling stage freezing tolerance determination method

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Embodiment 1

[0020] Embodiment 1: a kind of seedling stage frost tolerance identification method that is used for potato, it comprises the steps:

[0021] 1. Take the mature leaves and wash them with distilled water with ice, put the leaves into the bottom of a test tube (25×150mm) with a cover, put one leaf in each tube (24 pieces in total), with the leaf face down, and number each tube;

[0022] 2. Put the control test tube into the ice immediately, and put the rest of the test tubes into the water bath in turn (the medium in the water bath is a 1:1 mixture of ethylene glycol and distilled water);

[0023] 3. Set the initial temperature of the water bath to 0°C, keep it for 30 minutes, then drop it to -0.5°C and keep it for 30 minutes;

[0024] 4. Drop the temperature from -0.5°C to -1°C and keep it for 1h. When -1°C is kept for 30 minutes, add a small piece of ice to each tube, keep it for another 30 minutes and then lower it to -1.5°C, and keep it for 1h at -1.5°C;

[0025] 5. After t...

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Abstract

The invention discloses a potato seedling stage freezing tolerance determination method. The potato seedling stage freezing tolerance determination method comprises the following steps of, firstly, washing samples with iced distilled water and numbering the samples in test tubes; secondly, placing a contrast test tube into ice and the others test tubes into water bath; thirdly, slowly cooling downthe test tubes in water bath for freezing; fourthly, slicing unfrozen leaves, containing leaf slices into test tubes and filling in distilled water; fifthly, pumping air out of the test tubes througha vacuum pump, oscillating the test tubes on an oscillator, and standing the test tubes for measurement of a conductivity R1; sixthly, sterilizing the test tubes into a boiler, and cooling the test tubes down for measurement of a conductivity R2; seventhly, plugging the average value of 3 electrolyte permeation rates corresponding to every temperature into an equation for nonlinear fitting, taking the inflection point of the equation as a median lethal temperature point of a material to obtain the freezing tolerance of the material. The potato seedling stage freezing tolerance determination method can help rapidly determine the freezing tolerance of potatoes of different gene types without time and season restrictions, and meanwhile is accurate and reliable in data to provide important data for potato freezing tolerance research and achieve good technical effects.

Description

technical field [0001] The invention relates to a potato, in particular to a method for identifying the freezing resistance of the potato at the seedling stage, and belongs to the technical field of agricultural production. Background technique [0002] There is no research report on the frost tolerance of potatoes in China, and the identification methods of potato frost tolerance in foreign countries are artificial freezing identification and field frost identification. Artificial freezing identification is simple, inexpensive and the results are relatively accurate. Chen (1999) evaluated the freezing resistance of 20 different kinds of potato materials. The LT 50 Values ​​are accurate to 1 decimal place. At the same time, artificial freezing identification is not limited by seasons, and can quantify the frost tolerance of potato materials, and can simultaneously reflect the frost tolerance of the material before cold acclimation, the frost tolerance after cold acclimation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/00
CPCG01N25/00
Inventor 李飞金黎平雷尊国卞春松徐建飞段绍光罗小波尹旺
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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