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Method for rescuing early maturing flat peach embryo

An embryo rescue and pan peach technology, which is applied in the field of plant breeding, can solve the problems of less research on pan peach, unsuitable pan peach embryo rescue, etc., and achieves the effects of simple operation, improved breeding efficiency, and reduced fruit cracking rate.

Active Publication Date: 2019-03-19
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the traditional embryo rescue techniques are related to round peaches, and there are few studies on flat peaches, so they are not suitable for embryo rescue of flat peaches.

Method used

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  • Method for rescuing early maturing flat peach embryo
  • Method for rescuing early maturing flat peach embryo
  • Method for rescuing early maturing flat peach embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Rain shelter facilities: single greenhouse

[0048] Early-maturing flat peach mother plant: 'Hupan No. 1', a flat peach variety selected by the Shanghai Academy of Agricultural Sciences. The flowering period is March 24, and the fruit ripening period is mid-July. It is an excellent early-maturing flat peach variety.

[0049] Sire: 'Huyou 005'

[0050] test group:

[0051] After cross-pollination in a single greenhouse, the mother plant is managed. In mid-July, spray paclobutrazol 150-250 times liquid to inhibit the growth of shoots and promote the formation of flower buds. The amount of spray liquid is based on the dripping water on the leaves;

[0052] Spray paclobutrazol 150-250 times liquid according to the tree vigor during the dew red stage and 40 days after full flowering, and the amount of spray liquid is based on the dripping water on the leaves;

[0053] During the flowering stage and the young fruit stage, the temperature is regulated by switching the shed f...

Embodiment 2

[0078] Rain shelter facilities: single greenhouse

[0079] Early-maturing flat peach mother plant: 'Hupan No. 1', a flat peach variety selected by the Shanghai Academy of Agricultural Sciences. The flowering period is March 24, and the fruit ripening period is mid-July. It is an excellent early-maturing flat peach variety.

[0080] Sire: 'Huyou 005'

[0081] test group:

[0082] After cross-pollination in a single greenhouse, the mother plant is managed. In mid-July, spray paclobutrazol 150-250 times liquid to inhibit the growth of shoots and promote the formation of flower buds. The amount of spray liquid is based on the dripping water on the leaves;

[0083] Spray paclobutrazol 150-250 times liquid according to the tree vigor during the dew red stage and 40 days after full flowering, and the amount of spray liquid is based on the dripping water on the leaves;

[0084] During the flowering stage and the young fruit stage, the temperature is regulated by switching the shed f...

Embodiment 3

[0092] Rain shelter facilities: single greenhouse

[0093] Early-maturing flat peach mother plant: 'Hupan No. 1', a flat peach variety selected by the Shanghai Academy of Agricultural Sciences. The flowering period is March 24, and the fruit ripening period is mid-July. It is an excellent early-maturing flat peach variety.

[0094] Sire: 'Huyou 005'

[0095] After cross-pollination in a single greenhouse, the mother plant is managed. In mid-July, spray paclobutrazol 150-250 times liquid to inhibit the growth of shoots and promote the formation of flower buds. The amount of spray liquid is based on the dripping water on the leaves;

[0096] Spray paclobutrazol 150-250 times liquid according to the tree vigor during the dew red stage and 40 days after full flowering, and the amount of spray liquid is based on the dripping water on the leaves;

[0097] During the flowering stage and the young fruit stage, the temperature is regulated by switching the shed film to prevent the damag...

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Abstract

The invention provides a method for rescuing an early maturing flat peach embryo and belongs to the technical field of embryo rescuing. The method comprises the following steps: 1) after hybridizing an early maturing flat peach mother plant in a rain-sheltering facility, carrying out mother plant management; 2) spraying a 150 to 250 time solution of paclobutrazol within 40d after a red flower budforming period and a flowering period; 3) carrying out fruit thinning at a fruit core-hardening stage; 4) harvesting 4 to 8d before fruits become mature and obtaining in-vitro fruits; 5) separating the flat peach embryos from the in-vitro fruits in step 4) and inoculating the flat peach embryos into a WPM culture medium; culturing at 2 to 6 DEG C for 70 to 90d to obtain inoculated embryos; and 6)culturing the inoculated embryos under a light illumination condition of 18 to 25 DEG C and transplanting. The method provided by the invention has relatively high germination rate, seedling emergencyrate and transplanting survival rate.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to a method for rescuing early-maturing flat peach embryos. Background technique [0002] Pantao is a variety of peach, which is widely loved by people because of its unique flavor, strange appearance, convenient eating, and high edible rate. But the flat peach fruit is prone to rotten tops, is not resistant to storage, and the maturity period is relatively concentrated, which limits the development of the flat peach industry. Therefore, cultivating early-maturing and storage-resistant varieties is one of the important goals of current flat peach breeding. Early-maturing varieties have short fruit development period, incomplete seed development, and embryos are prone to abortion. The germination rate of flat peach seeds is very low when using conventional sowing methods. The use of embryo rescue technology can promote the continued development of immature embryos, which will...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G17/00A01G7/06
CPCA01G7/06A01G17/005A01H1/02
Inventor 杜纪红叶正文苏明申周慧娟李雄伟张夏南
Owner SHANGHAI ACAD OF AGRI SCI
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