Bacillus amyloliquefaciens GUAL210 and application thereof

A technology of amylolytic spores and bacilli, applied in the direction of application, bacteria, fungicides, etc.

Active Publication Date: 2019-03-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, tobacco black shank and tobacco bacterial wilt often co-occur in Guizhou, and there is no report on the screening of Bacillus that can control these two diseases at the same time

Method used

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  • Bacillus amyloliquefaciens GUAL210 and application thereof
  • Bacillus amyloliquefaciens GUAL210 and application thereof
  • Bacillus amyloliquefaciens GUAL210 and application thereof

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Experimental program
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Embodiment Construction

[0017] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0018] 1. Isolation and screening of Bacillus amyloliquefaciens GUAL210

[0019] 1. Materials and methods

[0020] 1.1 Materials

[0021] Soil samples were collected from the plots of tobacco black shank and bacterial wilt in Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018. The 5-point sampling method was used to collect soil samples from the same plot. Mixing counts as 1 soil sample. Use paper bags to collect soil samples, and disinfect soil collection tools in advance to avoid mixing soil microorganisms.

[0022] 1.2 Medium

[0023] LB medium is used for the isolation and preservation of Bacillus and plate confrontation experiments; PDA medium is mainly used for plate confrontation experiments.

[0024] 1.3 Tobacco disease strains

[0025] The tested strains of tobacco black shank (Phytophthora nicotianae) and tobacco bacterial wi...

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Abstract

The invention discloses bacillus amyloliquefaciens GUAL210, which is collected in the China Center for Type Culture Collection, with the collection number of CCTCC NO: M 2018871. The invention furtherdiscloses application of the bacillus amyloliquefaciens GUAL210 to simultaneous control of tobacco black shank and tobacco bacterial wilt. The bacillus amyloliquefaciens GUAL210 has an obvious ability of antagonizing both phytophthora nicotianae and ralatonia solanacearum simultaneously and the ability of antagonizing pepper anthracnose.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens GUAL210 and an application thereof, belonging to the technical field of biological control. Background technique [0002] Tobacco black shank and tobacco bacterial wilt are the most destructive soil-borne diseases in my country's tobacco production, causing huge economic losses every year. Guizhou Province is one of the main production areas of tobacco production in my country, and the occurrence of tobacco soil-borne diseases is particularly Seriously, the annual economic loss caused by the disease is nearly 100 million yuan, seriously affecting the economic income of tobacco farmers. At present, the prevention and control of this disease in production mainly adopts the comprehensive control measures of planting resistant (tolerant) varieties and cultivation control, supplemented by chemical control, but there is still no suitable excellent variety resistant (resistant) to soil-borne diseases in act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/07
CPCA01N63/00C12R2001/07C12N1/205Y02A50/30
Inventor 丁海霞彭丽娟余水
Owner GUIZHOU UNIV
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