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Bacillus amyloliquefaciens GUAL203 and application thereof

A technology for dissolving amyloid spores and bacilli, applied in the fields of application, bacteria, fungicides, etc., can solve the problems of pesticide residue environment, increase of field dosage, pollution and other problems

Active Publication Date: 2019-05-17
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, tobacco black shank and tobacco bacterial wilt often co-occur in Guizhou, and there is no report on the screening of Bacillus that can control these two diseases at the same time
[0004] In addition, capsicum anthracnose is one of the main diseases in capsicum production, which can cause damage throughout the growth period of capsicum, causing great losses
Guizhou Province is one of the main cultivation areas of pepper in my country. The long-term continuous cropping of pepper has caused the continuous expansion of the incidence of anthracnose, seriously affecting the income of farmers
At present, chemical pesticides are mainly used to prevent and control diseases in production. The resistance of pathogenic bacteria is increasing year by year, and the amount of pesticides used in the field is also increasing accordingly, which can easily cause pesticide residues and environmental pollution.

Method used

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  • Bacillus amyloliquefaciens GUAL203 and application thereof
  • Bacillus amyloliquefaciens GUAL203 and application thereof
  • Bacillus amyloliquefaciens GUAL203 and application thereof

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Embodiment Construction

[0018] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0019] 1. Isolation and screening of Bacillus amyloliquefaciens GUAL203

[0020] 1. Materials and methods

[0021] 1.1 Materials

[0022] Soil samples were collected from the plots of tobacco black shank and bacterial wilt in Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018. The 5-point sampling method was used to collect soil samples from the same plot. Mixing counts as 1 soil sample. Use paper bags to collect soil samples, and disinfect soil collection tools in advance to avoid mixing soil microorganisms.

[0023] 1.2 Medium

[0024] LB medium is used for the isolation and preservation of Bacillus and plate confrontation experiments; PDA medium is mainly used for plate confrontation experiments.

[0025] 1.3 Tobacco disease strains

[0026] The tested strains of tobacco black shank (Phytophthora nicotianae) and tobacco bacterial wi...

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Abstract

The invention discloses a bacillus amyloliquefaciens GUAL203 preserved at the China Center for Type Culture Collection under the accession number CCTCC NO: M 2018870. The invention also discloses an application of the bacillus amyloliquefaciens GUAL203 in simultaneously controlling tobacco black shank and tobacco bacterial wilt. The bacillus amyloliquefaciens GUAL203 has the obvious capability ofsimultaneously antagonizing tobacco black shank germs and tobacco bacterial wilt germs and the capability of antagonizing pepper anthracnose.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens GUAL203 and an application thereof, belonging to the technical field of biological control. Background technique [0002] Tobacco black shank and tobacco bacterial wilt are the most destructive soil-borne diseases in my country's tobacco production, causing huge economic losses every year. Guizhou Province is one of the main production areas of tobacco production in my country, and the occurrence of tobacco soil-borne diseases is particularly Seriously, the annual economic loss caused by the disease is nearly 100 million yuan, seriously affecting the economic income of tobacco farmers. At present, the prevention and control of this disease in production mainly adopts the comprehensive control measures of planting resistant (tolerant) varieties and cultivation control, supplemented by chemical control, but there is still no suitable excellent variety resistant (resistant) to soil-borne diseases in act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/07
Inventor 彭丽娟丁海霞余水
Owner GUIZHOU UNIV
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