Detection system used for simultaneously detecting lipoprotein sub-fraction cholesterol and lipoprotein particle concentration

A lipoprotein particle and detection system technology, applied in the field of detection system for simultaneous detection of lipoprotein subcomponent cholesterol and lipoprotein particle concentration

Inactive Publication Date: 2019-08-09
江西美康盛德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, in the existing invention, there is no method that can simultaneously detect the ...

Method used

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  • Detection system used for simultaneously detecting lipoprotein sub-fraction cholesterol and lipoprotein particle concentration
  • Detection system used for simultaneously detecting lipoprotein sub-fraction cholesterol and lipoprotein particle concentration
  • Detection system used for simultaneously detecting lipoprotein sub-fraction cholesterol and lipoprotein particle concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Comparison of sample test results

[0056] Take 50ul of serum, add 1950ul of KBr (density adjusted to 1.21) to dilute, and mix well. Take a 5.1ml Polyallomer quick-seal tube, add 3800ul of normal saline, and then slowly add 1200ul of the pre-diluted serum sample from the bottom. The centrifuge tube was placed in a rotor VTi-65.2 rotor and centrifuged with a Beckman ultracentrifuge. Parameters: speed = 65000rpm, ω 2 t=6.6*10 10 rad 2 / sec, temperature=23°C, acceleration=6, deceleration=6.

[0057] The centrifuged sample is carefully moved to the above-mentioned detection system for simultaneous detection of lipoprotein subcomponent cholesterol and lipoprotein particle concentrations of the present invention for detection. The detection parameters are: sample flow rate 1.0ml / min, reagent flow rate 1.0ml / min min. Wherein, the detection is that the reagents used are as shown in Table 1 below:

[0058] Table 1 Reagent R1 component content

[0059]

[0060]

[0...

Embodiment 2

[0067] Precision experiment

[0068] Take a sample, use this detection system, repeat the detection 10 times, and calculate the precision of the lipoprotein particle concentration, the results are shown in Table 3.

[0069] Table 3 Precision

[0070] project name Repeatability (CV) HDL-P 3.8％ LDL-P 2.6％ Lpa-P 5.7％ IDL-P 4.6％ VLDL-P 4.2％

[0071] It can be seen that the detection system has good precision in detecting the concentration of several different lipoprotein particles. Since the detection system only needs one detection, it can detect lipoprotein cholesterol and particle concentration of lipoprotein, and has good performance, so it has a good clinical application prospect.

Embodiment 3

[0073] linear experiment

[0074] Take a high-value sample for each item, dilute it with water into different concentration gradients, use this detection system for detection, and analyze how much its linearity can achieve. The specific results are shown in Table 4.

[0075] Table 4 Linear range

[0076]

[0077]

[0078] It can be seen that the linear range of these lipoprotein particle concentration items can meet the index requirements and cover the range required for clinical testing. Within this linear range, it has good linearity.

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Abstract

The invention relates to a detection system used for simultaneously detecting lipoprotein sub-fraction cholesterol and lipoprotein particle concentration. The detection system is characterized in thatthe system comprises a sample container, a reagent container, a cleaning fluid container, a reaction module, a first detection module and a second detection module; the sample container is connectedto the inlet pipeline of the first detection module through a pipeline; the cleaning liquid container is connected to a pipeline which is connected with the sample container and the first detection module through a pipeline; the outlet of the first detection module is connected with the inlet of the reaction module through a pipeline; the reagent container is connected to a pipeline which is connected with the first detection module and the reaction module through a pipeline; and the outlet end of the reaction module is connected with the second detection module. With the detection system adopted, lipoprotein cholesterol sub-fractions can be detected, and lipoprotein particle concentration can be also detected; and since the detection system does not need to perform detection twice, and time and cost are saved.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and specifically relates to an instrument and a reagent system that can be used to detect lipoprotein subcomponent cholesterol in serum samples and detect the particle concentration of lipoprotein components in serum samples. Assay system for the detection of lipoprotein subfraction cholesterol and lipoprotein particle concentrations. Background technique [0002] As we all know, lipoproteins can be divided into chylomicrons (CM), very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins according to their density. protein (HDL). Clinically, the cholesterol content of various lipoproteins is usually measured to guide the diagnosis. At present, not only total cholesterol (T-CH), but also LDL-C and HDL-C are routinely measured clinically. Among them, the high content of LDL-C can easily lead to atherosclerosis, c...

Claims

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Application Information

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IPC IPC(8): G01N21/51G01N21/31G01N21/15G01N15/06
CPCG01N21/51G01N21/31G01N21/15G01N15/06G01N2015/0693
Inventor 邹炳德邹继华汪屹贾江花徐勇
Owner 江西美康盛德生物科技有限公司
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