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A reagent for detecting the concentration of lipoprotein particles and its application method

A technology of lipoprotein particles and lipoproteins, applied in particle suspension analysis, measuring devices, suspension and porous material analysis, etc., can solve problems affecting precision and poor precision

Active Publication Date: 2022-02-18
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even seriously affect the precision of LDL-P, IDL-P, VLDL-P and other items
Therefore, whether it is the single detection of lipoprotein particle concentration in patent US9239280B2, or the simultaneous detection of lipoprotein cholesterol and lipoprotein particle concentration in CN110108673A, it shows poor precision and is more susceptible to the influence of high triglyceride samples

Method used

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  • A reagent for detecting the concentration of lipoprotein particles and its application method
  • A reagent for detecting the concentration of lipoprotein particles and its application method
  • A reagent for detecting the concentration of lipoprotein particles and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Reagent preparation and detection steps

[0038] Take 50ul of serum, add 1950ul of diluent (see Table 1 below), and mix well. Take a 5.0ml Polyallomer quick-seal tube, add 3800ul of density solution (see Table 1 below), and then slowly add 1200ul of the pre-diluted serum sample from the bottom. The centrifuge tube was placed in a rotor VTi-65.2 rotor and centrifuged with a Beckman ultracentrifuge. Parameters: rotating speed=65000rpm, time (see table below), temperature=23°C, acceleration=6, deceleration=6.

[0039] Carefully move the centrifuged sample to the lipoprotein particle concentration detection instrument for detection. The buffer used in the detection on the machine is 20mM phosphate buffer, pH 7.0.

[0040] Table 1. Formulas of different combinations

[0041]

[0042]

[0043]

[0044] The concentration of some substances in the above table is not indicated, such as KBr, which is used to adjust the density of the diluent. According to the density...

Embodiment 2

[0046] Precision experiment

[0047] Take a sample, use this detection system, repeat detection 10 times, calculate the precision of lipoprotein particle concentration, its result is shown in Table 2, the sample formula that adopts is the formula configured in the embodiment, as formula 1 in Table 2 Corresponding to formula 1 in Table 1 (components: diluent KBr, density 1.21g / ml; density solution NaCl, 0.1mM EDTA, 0.1% ethanol, density 1.05g / ml; centrifugation time 30min), the same below.

[0048] Table 2 Precision (sample 1, TG concentration: 1.5mM)

[0049]

[0050]

[0051]

[0052] Table 3 Precision (sample 2, TG concentration: 2.0mM)

[0053]

[0054]

[0055]

[0056] Table 4 precision (sample 3, TG concentration: 3.2mM)

[0057]

[0058]

[0059]

[0060] Table 5 precision (sample 4, TG concentration: 4.5mM)

[0061]

[0062]

[0063]

[0064] Table 6 precision (sample 5, TG concentration: 6.2mM)

[0065]

[0066]

[0067] ...

Embodiment 3

[0070] linear experiment

[0071] Take a high-value sample for each item, dilute it with water into different concentration gradients, and use this detection system for detection to verify its linear range. The specific linear ranges of the verified items are HDL-P: 1-80umol / L; LDL-P: 20-6000nmol / L; Lpa-P: 2-300nmol / L; IDL-P: 1-100nmol / L; VLDL-P: 2-500nmo / L. See Table 3 for the specific results of the judgment dilution R2 of the linear range verification.

[0072] Table 7 Linear range verification results

[0073]

[0074]

[0075]

[0076] It can be seen that the linear range of these lipoprotein particle concentration items can meet the index requirements and cover the range required for clinical testing. Within this linear range, it has good linearity.

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Abstract

The invention discloses a reagent for detecting the concentration of lipoprotein particles. The reagent is composed of the following components: a diluent, a density liquid, and a buffer, and the density liquid contains alcohols, and the alcohols The concentration of the substance is 0.1%‑50%. The invention belongs to the field of in vitro diagnosis. The invention provides a reagent for detecting the concentration of lipoprotein particles. The reagent can detect the concentration of protein particles alone, and can be used in combination with other instrument reagents to simultaneously detect lipoprotein cholesterol subcomponents without having to detect twice, saving It saves time and cost, has high commercial value, and can be widely used in clinical tests and scientific research experiments.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, in particular to a reagent that can be used to detect the particle concentration of lipoprotein components in serum samples. Background technique [0002] As we all know, lipoproteins can be divided into chylomicrons (CM), very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins according to their density. protein (HDL). Clinically, the cholesterol content of various lipoproteins is usually measured to guide the diagnosis. At present, not only total cholesterol (T-CH), but also LDL-C and HDL-C are routinely measured clinically. Among them, the high content of LDL-C can easily lead to atherosclerosis, commonly known as bad cholesterol, while HDL-C has a certain protective effect on blood vessels, commonly known as good cholesterol. However, recent studies have shown that both LDL-C and HDL-C are heterogeneous, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/06
CPCG01N15/06G01N2015/0693G01N33/92
Inventor 邹炳德汪屹贾江花
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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