In-vitro hatching method for cherax quadricarinatus embryos
A technology for red crayfish and embryos, which is applied in the directions of climate change adaptation, fish farming, application, etc., can solve the problems of inability to apply large-scale seedlings of Crayfish, low hatching rate in vitro, low hatching rate of embryos, etc. Achieve the effect of high hatching rate and high synchronization rate
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Embodiment 1
[0024] The in vitro hatching method of red crayfish embryos includes: (1) taking red crayfish embryos that are in the middle stage of development and appearing orange-yellow; (2) gently peeling off the embryos along the abdomen with a comb, and using sterilized formaldehyde solution for 10 seconds, and then washed with sterile water; (3) hatch the washed embryos, control the hatching water temperature at 30°C, and change the water every 3 days; (4) continue to cultivate the embryos after hatching, When the yolk sacs of some shrimps disappeared, start feeding Artemia once a day; until the yolk sacs of all the shrimps disappeared, shrimp seedlings were obtained.
Embodiment 2
[0026] The in vitro hatching method of red crayfish embryos includes: (1) taking the red crayfish embryos that are in the middle stage of development and showing orange-yellow eggs; (2) gently peeling off the embryos along the abdomen with a comb, and using The formaldehyde solution was disinfected for 10 seconds, and then washed with sterile water; (3) The washed embryos were hatched, and the hatching water temperature was controlled at 30°C, and the water was changed every 2 days; When some shrimp yolk sacs were found to disappear, start feeding Artemia once a day; until all shrimp yolk sacs disappeared, shrimp seedlings were obtained.
Embodiment 3
[0028] The in vitro hatching method of red crayfish embryos includes: (1) taking red crayfish embryos that are in the middle stage of development and showing orange-yellow eggs; (2) gently peeling off the embryos along the abdomen with a comb, and using The formaldehyde solution was disinfected for 10 seconds, and then washed with sterile water; (3) The washed embryos were hatched, and the hatching water temperature was controlled at 29°C, and the water was changed every 2 days; (4) After the embryos were hatched, continue to be cultivated. When some shrimp yolk sacs were found to disappear, start feeding Artemia once a day; until all shrimp yolk sacs disappeared, shrimp seedlings were obtained.
[0029] Embodiment 1-3 step (3) and (4) the incubation device that adopts is as attached Figure 3-4 As shown, it includes an incubator (including temperature control rod) (not shown in the figure), an incubator installed on the wall of the incubator through a suction cup, the incubat...
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