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In-vitro hatching method for cherax quadricarinatus embryos

A technology for red crayfish and embryos, which is applied in the directions of climate change adaptation, fish farming, application, etc., can solve the problems of inability to apply large-scale seedlings of Crayfish, low hatching rate in vitro, low hatching rate of embryos, etc. Achieve the effect of high hatching rate and high synchronization rate

Inactive Publication Date: 2019-09-24
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wang Qing (2012) studied the hatching of embryos stripped at different periods and the effects of different hatching methods after stripping on the hatching rate. The higher rate shows that the embryos of Procambarus clarkii can be separated and hatched separately, which further shows that the fertilized eggs of crustaceans can hatch without the mother, but the in vitro hatching rate is low, so it cannot be applied to Procambarus clarkii. Shrimp large-scale nursery

Method used

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  • In-vitro hatching method for cherax quadricarinatus embryos

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The in vitro hatching method of red crayfish embryos includes: (1) taking red crayfish embryos that are in the middle stage of development and appearing orange-yellow; (2) gently peeling off the embryos along the abdomen with a comb, and using sterilized formaldehyde solution for 10 seconds, and then washed with sterile water; (3) hatch the washed embryos, control the hatching water temperature at 30°C, and change the water every 3 days; (4) continue to cultivate the embryos after hatching, When the yolk sacs of some shrimps disappeared, start feeding Artemia once a day; until the yolk sacs of all the shrimps disappeared, shrimp seedlings were obtained.

Embodiment 2

[0026] The in vitro hatching method of red crayfish embryos includes: (1) taking the red crayfish embryos that are in the middle stage of development and showing orange-yellow eggs; (2) gently peeling off the embryos along the abdomen with a comb, and using The formaldehyde solution was disinfected for 10 seconds, and then washed with sterile water; (3) The washed embryos were hatched, and the hatching water temperature was controlled at 30°C, and the water was changed every 2 days; When some shrimp yolk sacs were found to disappear, start feeding Artemia once a day; until all shrimp yolk sacs disappeared, shrimp seedlings were obtained.

Embodiment 3

[0028] The in vitro hatching method of red crayfish embryos includes: (1) taking red crayfish embryos that are in the middle stage of development and showing orange-yellow eggs; (2) gently peeling off the embryos along the abdomen with a comb, and using The formaldehyde solution was disinfected for 10 seconds, and then washed with sterile water; (3) The washed embryos were hatched, and the hatching water temperature was controlled at 29°C, and the water was changed every 2 days; (4) After the embryos were hatched, continue to be cultivated. When some shrimp yolk sacs were found to disappear, start feeding Artemia once a day; until all shrimp yolk sacs disappeared, shrimp seedlings were obtained.

[0029] Embodiment 1-3 step (3) and (4) the incubation device that adopts is as attached Figure 3-4 As shown, it includes an incubator (including temperature control rod) (not shown in the figure), an incubator installed on the wall of the incubator through a suction cup, the incubat...

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Abstract

The invention belongs to the technical field of aquaculture, and discloses an in-vitro hatching method for cherax quadricarinatus embryos. The method comprises the steps that 1, ovigerous cherax quadricarinatus with the embryos in the mid-development stage and in orange yellow is obtained; 2, the embryos are slightly stripped along pleopods by using a comb, disinfected by using a disinfectant and washed with sterile water; 3, the washed embryos are hatched, the hatching water temperature is controlled to be 29-30 DEG C, water is replaced every 2-3 days, and dead embryos or dead shrimps are removed in time; 4, after being hatched, the embryos continue to be bred, when it is found that part of shrimp egg yolk sacs disappear, feeding is started, and feeding is carried out once every day until all the shrimp egg yolk sacs disappear to obtain shrimp seeds. The method greatly improves the egg carrying and hatching effects of parent cherax quadricarinatus, the hatching synchronism rate is relatively high, and a reference is provided for exploration and establishment of a breeding technology of the cherax quadricarinatus SPF seeds.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the cultivation of red-clawed crayfish, in particular to a method for in vitro hatching of red-clawed crayfish embryos. Background technique [0002] Red Claw Crawfish ( Cherax quadricariratus ), also known as Australian freshwater lobster, is native to Australia and other regions, and belongs to Crustaoea, Decapoda, Parastacidae, and Cherax. It is one of the world's rare and economical shrimps due to its strong resistance, tender meat and high meat yield. In recent years, with the decline of domestic vannamei breeding benefits, the market demand for crayfish and other crayfish has increased sharply, and the industrial scale of red crayfish has also been rapidly expanded. Another emerging large-scale breeding species. [0003] Since the introduction of red-clawed crayfish into China, a series of studies have been carried out on broodstock cultivation, artificia...

Claims

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Application Information

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IPC IPC(8): A01K61/17A01K63/04
CPCA01K61/17A01K63/04A01K63/042Y02A40/81
Inventor 程顺顾志敏贾永义迟美丽刘士力郑建波
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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