A rapid and high-efficiency propagation method for in vitro induction of bulbil formation by Lily Capsulata stems
A technology of lily lily lily and bulbils, which is applied in the field of rapid and efficient propagation of edible and medicinal lily lily lily stems to induce bulbil formation in vitro, and can solve the problems of high reproduction coefficient and the reproductive cycle of lily lily bulbs susceptible to environmental influences, etc. Achieve the effects of increasing the reproduction coefficient, shortening the cultivation time, and strong controllability
Active Publication Date: 2021-04-06
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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[0006] Aiming at the advantages of the high reproductive coefficient of Lily Lily Buds and the disadvantages that the Lily Lilium Bud formation is easily affected by the environment and the breeding cycle is long under natural conditions, the present invention aims to provide a rapid breeding method for in vitro induction of Lily Lily Lily Bud formation. method, in order to shorten the formation and cultivation time of Capsulata bulbils, and improve the reproduction efficiency and reproduction coefficient
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Embodiment 1
[0030] Embodiment 1 A kind of rapid and high-efficiency propagation method for in vitro induction of bulbil formation by lily lily lily stem section, comprising the following steps:
[0031] (1) Obtaining and surface sterilization of explants: the freshly harvested Julienne bulbs were embedded in the matrix and refrigerated in a 4°C cold storage until they germinated until the buds were 10 cm long ( figure 1 a), peel off the scales, cut off the stem buds from the bulb base with a double-sided blade, get 10cm long, and the stem buds wrapped by leaves ( figure 1 b) Wash away the substrate under running water, wash with detergent water for 10 minutes, rinse under running water until no foam is produced, then put it into a jar, and perform surface sterilization on an ultra-clean workbench. Surface sterilization: first sterilize the stem buds with 75% alcohol for 30 seconds, wash them once with sterile water, then sterilize them with 10% NaClO for 5 minutes, and finally wash them 4...
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The invention provides a rapid and high-efficiency propagation method for in vitro induction of bulbil formation by the stem section of lily lily lily. The steps are: (1) precooling treatment of the bulb; (2) elongation culture of the stem; (3) induction of bulbil by the stem section Formation, bulbil expansion and rooting. The medium for elongating the stems of Chinese herb is: MS+sucrose 30g / L+agar 5.0g / L; the medium for bulbil induction, expansion and rooting is: MS+6-BA 1.0mg / L+sucrose 30g / L+ Agar 5.0g / L. In the method for in vitro induction of bulbil formation and propagation of the lilac lily of the present invention, the elongated stem can produce about 20 or more node leaf axils, and each leaf axil can form 2 to 3 bulbils, which significantly improves the reproduction coefficient and efficiency of the lily lily. Compared with the process of bulbil formation and scale direct induction of clustered buds to produce bulblets under natural conditions, the breeding cycle is greatly shortened, steps are simplified, and it has important practical application value.
Description
technical field [0001] The invention belongs to the technical field of flower cultivation, and relates to a rapid and high-efficiency propagation method for in vitro induction of bulbil formation by edible and medicinal Lilium lancifolium stem segments. Background technique [0002] Lilium lancifolium, also known as Yixing lily and tiger skin lily, is a perennial bulbous flower of the genus Lilium in the family Liliaceae (Long Yayi, Zhang Jinzheng, Zhang Lannian. Lily—the king of bulbous flowers. Beijing: Golden Shield Publishing House, 1999.). Its flowers are large and colorful, with strong resistance, and have high ornamental, edible and medicinal values. It is also an excellent parent of lily breeding (Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China. Vol. 14. Beijing: Science Press ,1980.; National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China. Beijing: China Medical Science and Technology Press, 2015.; Yu X R,...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 明军杨盼盼徐雷锋何国仁唐玉超
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI