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Rhipicephalus haemaphysaloides vacuolar protein sorting molecule and application thereof

A technology of sorting protein and Rhizocephalus, applied in the field of bioengineering, can solve the problems of tick research without literature reports, achieve the effect of reducing the upper body rate and broad application prospects

Active Publication Date: 2019-12-06
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

VPS28 has been widely studied in other species, but there is no literature report on the study in ticks

Method used

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  • Rhipicephalus haemaphysaloides vacuolar protein sorting molecule and application thereof
  • Rhipicephalus haemaphysaloides vacuolar protein sorting molecule and application thereof
  • Rhipicephalus haemaphysaloides vacuolar protein sorting molecule and application thereof

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Effect test

Embodiment 2

[0040] Embodiment 2 RT-qPCR detects the expression distribution of Rhizocephalus falciparum VPS28 gene

[0041] Primers for fluorescent quantitative PCR were designed according to the ORF sequence of the VPS28 gene, and the primer sequences were as follows:

[0042]VPS28-qRTPCR-F: 5'-CCAGTACAAGGCAGCATTCAAG-3' (SEQ ID NO.5);

[0043] VPS28-qRTPCR-R:5'-GATTCGTTCCATGGCAGCAG-3' (SEQ ID NO. 6).

[0044] Primers were designed according to the full-length sequence of the internal reference gene as follows:

[0045] ELFIA-QRT-PCR-F: 5'-CGTCTACAAAGATTGGTGGCATT-3' (SEQ ID NO.7);

[0046] ELFIA-QRT-PCR-R: 5'-CTCAGTGGTCAGGTTGGCAG-3' (SEQ ID NO. 8).

[0047] The reaction system of fluorescent quantitative PCR was operated according to the kit instructions, and the cDNA obtained by reverse transcription of different materials of Rhizocephalus falciparum was used as a template for two-step real-time quantitative PCR analysis. According to Takara's operating instructions, the ABI7500 Real...

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Abstract

The invention discloses rhipicephalus haemaphysaloides vacuolar protein sorting molecule. The molecule has an amino acid sequence shown as SEQ ID NO. 1. The invention also discloses a gene of the rhipicephalus haemaphysaloides vacuolar protein sorting molecule, wherein the gene comprises a nucleotide sequence for encoding an amino acid sequence shown in the SEQ ID NO.1. According to the rhipicephalus haemaphysaloides vacuolar protein sorting molecule and the genes thereof, after gene silencing or knockout, body landing rate of ticks is obviously reduced, the blood absorption of the ticks is remarkably influenced, the death rate of the ticks reaches 100%, and the molecule is expected to become a candidate molecule for tick-resistant vaccines and tick-borne disease prevention and treatment,and has wide application prospects.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a vacuolar sortilin molecule of Rhizocephalus falciparum and an application thereof. Background technique [0002] Ticks are a class of non-permanent parasites that live exclusively on the surface of vertebrates and are the second largest vector of transmission in the world after mosquitoes. Ticks bite hosts and transmit pathogens to hosts, causing harm to hosts, seriously hindering the development of animal husbandry, and causing great economic losses. Ticks can also transmit various pathogens to humans, such as Lyme disease, hemorrhagic fever and novel Bria virus disease, etc., causing systemic infection, and severe cases can cause human death due to multiple organ failure. However, the current methods of preventing tick bites are relatively limited, mainly chemical drugs to repel ticks, which cannot fundamentally solve ticks and tick-borne diseases, and are prone to dr...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/113A61K39/00A61P33/14A01N57/16A01P7/00
CPCC07K14/43527C12N15/113A61P33/14A01N57/16C12N2310/14A61K39/00
Inventor 周金林王方方旷策嫣龚海燕张厚双曹杰周勇志
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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