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Rhipicephalus haemaphysaloides clathrin heavy-chain molecule and application thereof

A technology of clathrin and R. tick, applied in the field of bioengineering, can solve the problem of lack of literature reports in research, and achieve the effect of reducing the upper body rate and having broad application prospects.

Active Publication Date: 2019-12-06
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, the clathrin-mediated endocytic pathway has been extensively studied in other species such as Drosophila, but the clathrin heavy chain (CHC) gene in ticks has not been reported in the literature.

Method used

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  • Rhipicephalus haemaphysaloides clathrin heavy-chain molecule and application thereof
  • Rhipicephalus haemaphysaloides clathrin heavy-chain molecule and application thereof
  • Rhipicephalus haemaphysaloides clathrin heavy-chain molecule and application thereof

Examples

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Embodiment 2

[0045] Example 2 RT-qPCR detection of dynamic distribution of clathrin heavy chain CHC gene in R. falciformis

[0046] According to the ORF sequence of the clathrin heavy chain gene, the primers for fluorescence quantitative PCR are designed, and the primer sequences are as follows:

[0047] CHC-qRTPCR-F: 5'-CTACGAGTGCTTTGGTGCCT-3' (SEQ ID NO. 7);

[0048] CHC-qRTPCR-R: 5'-GTGATGTATTCCCGCATGACCT-3' (SEQ ID NO. 8).

[0049] The primers are designed according to the full-length sequence of the internal reference gene as follows:

[0050] ELFⅠA-QRT-PCR-F: 5'-CGTCTACAAGATTGGTGGCATT-3' (SEQ ID NO. 9);

[0051] ELFIA-QRT-PCR-R: 5'-CTCAGTGGTCAGGTTGGCAG-3' (SEQ ID NO. 10).

[0052] The fluorescence quantitative PCR reaction system was operated in accordance with the kit instructions, and the cDNA obtained by reverse transcription of different materials from R. falciparum was used as a template to perform a two-step real-time quantitative PCR analysis. According to Takara's operating instructions...

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Abstract

The invention discloses a rhipicephalus haemaphysaloides clathrin heavy-chain molecule having an amino acid sequence as shown in a SEQ ID NO.1, and further discloses a rhipicephalus haemaphysaloides clathrin heavy-chain molecular gene having a nucleotide sequence encoding the amino acid sequence as shown in the SEQ ID NO.1. According to the rhipicephalus haemaphysaloides clathrin heavy-chain molecule and gene thereof, after the gene is silenced or knocked out, the rate of ticks on a body can be remarkably reduced, and hematophagia of ticks can be remarkably influenced, and the death rate of ticks is 100 percent. The rhipicephalus haemaphysaloides clathrin heavy-chain molecule is expected to become an anti-tick vaccine and a candidate molecule for prevention and treatment of tick-borne diseases, and has a broad application prospect.

Description

Technical field [0001] The present invention relates to the technical field of bioengineering, in particular to a clathrin heavy chain molecule of Rhipicephalus falciparum and its application. Background technique [0002] Ticks are a class of non-permanent parasites that specifically parasitize the surface of vertebrates. They are the second largest vector in the world after mosquitoes. Ticks bite the host and spread the pathogen to the host, causing damage to the host, severely hindering the development of animal husbandry, and causing great economic losses. Ticks can also transmit various pathogens to humans, such as Lyme disease, hemorrhagic fever, and new Burya virus disease, causing systemic infections. In severe cases, multiple organ failure can cause death. However, the methods to prevent tick bites at this stage are relatively limited, mainly chemical drugs to repel ticks, which cannot fundamentally solve tick and tick-borne diseases, and are prone to drug resistance. T...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/113A61K31/713A61P33/14
CPCC07K14/43527C12N15/113A61K31/713A61P33/14C12N2310/14
Inventor 周金林王方方旷策嫣龚海燕张厚双曹杰周勇志
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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