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The vacuolar sortilin molecule of R. falciparum and its application

A technology of sorting protein and R. tick, applied in the field of bioengineering, can solve the problem of lack of literature reports in tick research, and achieve the effect of reducing the upper body rate and having broad application prospects.

Active Publication Date: 2022-07-15
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

VPS28 has been widely studied in other species, but there is no literature report on the study in ticks

Method used

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  • The vacuolar sortilin molecule of R. falciparum and its application
  • The vacuolar sortilin molecule of R. falciparum and its application
  • The vacuolar sortilin molecule of R. falciparum and its application

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Experimental program
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Effect test

Embodiment 2

[0040] Embodiment 2 RT-qPCR detects the expression distribution of R. falciparum VPS28 gene

[0041] The primers for fluorescence quantitative PCR were designed according to the ORF sequence of the VPS28 gene. The primer sequences are as follows:

[0042]VPS28-qRTPCR-F: 5'-CCAGTACAAGGCAGCATTCAAG-3' (SEQ ID NO. 5);

[0043] VPS28-qRTPCR-R: 5'-GATTCGTTCCATGGCAGCAG-3' (SEQ ID NO. 6).

[0044] Primers were designed according to the full-length sequence of the reference gene as follows:

[0045] ELFIA-QRT-PCR-F: 5'-CGTCTACAAGATTGGTGGCATT-3' (SEQ ID NO. 7);

[0046] ELFIA-QRT-PCR-R: 5'-CTCAGTGGTCAGGTTGGCAG-3' (SEQ ID NO. 8).

[0047] The reaction system of fluorescence quantitative PCR was operated according to the instructions of the kit, and the cDNA obtained by reverse transcription of different materials of R. falciparum was used as a template to carry out two-step real-time quantitative PCR analysis. According to Takara's operating instructions, the ABI7500 Real-time PCR sy...

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Abstract

The invention discloses a vacuolar sortilin molecule of R. falciparum, which has the amino acid sequence shown in SEQ ID NO.1. The invention also discloses the vacuolar sortilin molecular gene of R. falciparum, comprising a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO.1. The vacuolar sortilin molecule of R. falciparum and its gene of the present invention, after gene silencing or knockout, can significantly reduce the upper body rate of the tick, significantly affect the blood-sucking of the tick, and make the mortality rate of the tick reach 100%, which is expected to It has become a candidate molecule for anti-tick vaccine and tick-borne disease prevention and treatment, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a vacuolar sortilin molecule of R. falciparum and its application. Background technique [0002] Ticks are a class of non-permanent parasites that exclusively parasitize vertebrate bodies and are the second largest vector of transmission in the world after mosquitoes. Ticks bite the host and transmit pathogens to the host, causing damage to the host, seriously hindering the development of animal husbandry and causing great economic losses. Ticks can also transmit various pathogens to humans, such as Lyme disease, hemorrhagic fever, and novel Bria virus disease, causing systemic infection and, in severe cases, death due to multiple organ failure. However, the methods for preventing tick bites at this stage are relatively limited, mainly chemical drugs to drive ticks, which cannot fundamentally solve ticks and tick-borne diseases, and are prone to drug resistance. Therefore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C12N15/12C12N15/113A61K39/00A61P33/14A01N57/16A01P7/00
CPCC07K14/43527C12N15/113A61P33/14A01N57/16C12N2310/14A61K39/00
Inventor 周金林王方方旷策嫣龚海燕张厚双曹杰周勇志
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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