Method for establishing mouse model of obstructive sleep apnea with aortic dissection

Abstract

The present invention relates to the construction of an animal model, specifically a method for establishing a mouse model of obstructive sleep apnea with aortic dissection. In the method, a subcutaneous pump is used to continuously perfuse angiotensin II in an intermittent hypoxia simulation chamber to establish a mouse model. Animal model, the method of the present invention establishes a mouse model of obstructive sleep apnea with aortic dissection, which combines the IHR curve of an adult patient with simulated severe OSAS and the AD mouse model induced by Ang II to evaluate this new mouse model Finally, we were able to demonstrate that the IH group had a higher rate of AD formation, a larger maximum aortic diameter, and a lower cumulative survival rate than the control group. These phenotypic data are consistent with findings in humans. Therefore, we established a reliable and easy-to-use mouse model to study the role of OSAS in AD.

Description

technical field [0001] The invention relates to the construction of animal models, in particular to a method for establishing a mouse model of obstructive sleep apnea with aortic dissection. Background technique [0002] Aortic dissection refers to the sudden tearing of the aortic intima caused by various reasons, and the circulating blood enters the middle layer of the vessel wall and causes its stratification. Driven by the pressure of the blood flow, it gradually expands in the aortic media to form a dissection . Acute onset, sudden severe chest pain, shock and involvement of corresponding aortic branch vessels lead to acute ischemia of organs are the characteristics of the disease. In my country, with the aging of the population and the change of diet structure, the incidence of AD is rising rapidly. Epidemiology found that many cardiovascular risk factors such as smoking, male, age, hypertension and atherosclerosis are closely related to the pathogenesis of AD. Despi...

AI Technical Summary

Problems solved by technology

[0005] At present, there are many studies on aortic dissection models, including surgical production, drug stimulation and other methods, but there is still a lack of classic, unified and effective animal models of aortic dissection, especially those combined with OSA. Therefore, the impact of intermittent hypoxia The pathophysiological research on the formation of aortic dissection urgently needs experimental animal models that can simulate the pathological anatomy and pathophysiology of human aortic dissection with OSA

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