Preparation and separation and purification method of an immunomodulatory peptide

An immunomodulation and amino acid technology, applied in the biological field, can solve the problem of no relevant reports on the preparation of polypeptides, and achieve the effects of simple and efficient enzymatic hydrolysis technology and avoiding cost waste.

Active Publication Date: 2021-05-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been many studies on the chemical composition and medicinal efficacy of Pseudostellariae Pseudostellariae, but there is no relevant report on the preparation of polypeptides by proteolysis from Pseudostellariae Pseudostellariae

Method used

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  • Preparation and separation and purification method of an immunomodulatory peptide
  • Preparation and separation and purification method of an immunomodulatory peptide
  • Preparation and separation and purification method of an immunomodulatory peptide

Examples

Experimental program
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Embodiment 1

[0023] The amino acid sequence of the immunomodulatory peptide of the present invention is Tyr-Gly-Pro-Ser-Ser-Tyr-Gly-Tyr-Gly.

[0024] The preparation method is as follows:

[0025] Step 1: Grind Pseudostellariae Radix Pseudostellariae into powder with a traditional Chinese medicine grinder, pass through a 60-mesh sieve; weigh 5.0 g Pseudostellariae Pseudostellariae powder, add 100 mL of 0.3 mol / L sodium hydroxide solution, extract at 52.1 °C for 1.5 h, 10,000 After centrifuging at rpm for 20 min, take the supernatant, adjust the pH to 2.5 with 2 mol / L hydrochloric acid, let it stand for 1 h, centrifuge at 5,000 rpm for 20 min, take the precipitate, and freeze-dry it to obtain Pseudostellaria protein.

[0026] Step 2: Weigh 10.0 g of Pseudostellariae protein, add 100 mL of deionized water, adjust the pH to 2.0, add 0.8 g of pepsin, place it at 37 °C for 5.0 h, adjust the pH to 7.0, add 0.8 g of pancreatic Protease, placed in 37 ℃ for 3.0 h, boiled in a boiling water bath fo...

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Abstract

The invention provides a method for separating and purifying to obtain immunomodulatory peptides from Radix Radix, the method uses Radix Radix Astragali as a raw material, through protein extraction, double-step enzymolysis of pepsin and trypsin, separation and purification to obtain specific immune polypeptides, Its molecular weight is 949 Da, and its full amino acid sequence is: Tyr‑Gly‑Pro‑Ser‑Ser‑Tyr‑Gly‑Tyr‑Gly. The invention overcomes the shortcomings of the existing immunomodulatory drugs and eliminates the public's worries about artificial immunomodulators.

Description

technical field [0001] The invention relates to a method for separating and purifying immunoregulatory peptides from Radix Pseudostellariae, belonging to the field of biotechnology. Background technique [0002] Immunity is a specific physiological reaction that occurs when the body contacts "antigenic foreign bodies" or "foreign molecules", and its main purpose is to maintain the homeostasis of the body. The immune system is a network of cells, tissues, and organs that works to eliminate potentially harmful substances such as bacteria, viruses, fungi, protozoa, and to stop the growth of cancer cells. In the body's survival, the immune system acts as the first line of defense against pathogens, providing protection before bodily functions are impaired. However, the immune system is also affected by many factors, including stress, unhealthy lifestyle, pathogens and antigens, which can damage the body's immune system. As a result, drugs used to regulate the human immune resp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/20C07K1/16C07K1/14
CPCC07K7/06C12P21/06
Inventor 汪少芸杨倩蔡茜茜陈旭田永奇
Owner FUZHOU UNIV
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