Application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection

A technology of acetyltransferase and octenidine, applied in the field of pharmacy, can solve problems such as few protease studies

Inactive Publication Date: 2020-02-14
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are more than 100 proteases encoded by Mycobacterium tuberculosis, which play an important role in the growth cycle of Mycobacterium tuberculosis. However, there are few studies on proteases

Method used

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  • Application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection
  • Application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection
  • Application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0027] 1. Expression and purification of acetyltransferase (Eis) in Mycobacterium tuberculosis

[0028] Expressed according to literature (Chen W, Biswas T, Porter VR, Tsodikov OV, Garneau-Tsodikova S (2011) Unusual regioversatility of acetyltransferase Eis, a cause of drug resistance in XDR-TB.Proc Natl Acad Sci USA 108:9804–9808) and purified acetyltransferase (Eis)

[0029] (1) Transform the strain of Escherichia coli BL21 (Escherichia coli) (DE3) with the pET28a vector containing the Eis gene, and use LB plate medium (containing 50 mg / L kanamycin) to screen for positive clones.

[0030] (2) Pick positive clones on the LB plate culture medium, and transfer to 0.8L LB medium (containing 50mg / L kanamycin) after cultivating overnight at 37°C. When OD600) reached 0.6, 0.1 mM IPTG (Isopropylβ-D-Thiogalactoside) was added and cultured at 16° C. for 16 hours.

[0031] (3) Collect the cells by centrifugation at 5000rpm for 10min and then autoclave the bacteria; centrifuge the sol...

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Abstract

The embodiment of the invention provides application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection. The structure formula of the octenidine is as shown. Experiments show that the octenidine can well inhibit acetyltransferase and has a remarkable inhibition effect on mycobacterium tuberculosis and clinical mycobacterium tuberculosis wide drug-resistant strains499 and 421.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to the application of octenidine in inhibiting acetyltransferase and resisting mycobacterium infection. Background technique [0002] Tuberculosis (TB) is an infectious disease caused by Mycobacterium tubercuosis (Mtb). It is mainly transmitted through the respiratory tract and can cause symptoms such as cough, chest pain, hemoptysis, and dyspnea. It occurs when the body's immunity is weakened. According to estimates by the World Health Organization, despite vigorous efforts to curb tuberculosis infection worldwide, 10.4 million new patients develop tuberculosis (TB) each year, resulting in approximately 1.5 million deaths worldwide each year. In 1993, WHO declared a "global tuberculosis emergency", arguing that tuberculosis has become an important public health problem all over the world. [0003] Tuberculosis was discovered in 1882 by German microbiologist Robert Koch. Microsc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/444A61P31/04
CPCA61K31/444A61P31/04
Inventor 刘祥赵欣杨海涛陈榕朱倩张晶
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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