Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of GTF2IRD2 autoantibody detection reagent in preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of existing technologies such as no lung cancer, and achieve the effects of low patient harm, effective screening, and good application prospects

Active Publication Date: 2020-02-28
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF14 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] GTF2IRD2 (the gene sequence number is Ensembl: ENSG00000196275), there is no related report on GTF2IRD2 protein autoantibody, and there is no prior art related to lung cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of GTF2IRD2 autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of GTF2IRD2 autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of GTF2IRD2 autoantibody detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Relationship between GTF2IRD2 Autoantibodies in Plasma and Lung Cancer

[0028] 1. Clinical data

[0029] Select 30 cases of lung cancer patients, 29 cases of benign lung diseases (tuberculosis, hamartoma and other non-malignant tumors), and 30 cases of healthy controls. The basic information is as follows:

[0030]

[0031] 2. Detection principle

[0032] HuProt TM GTF2IRD2 protein is immobilized on the human protein custom chip (the GTF2IRD2 protein used is a full-length protein, and the UniProtKB number is: Q86UP8). Antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green), through The fluorescence scanner reads the signal, and the strength of the signal is positively correlated with the affinity and quantity of the antibody.

[0033] 3. Method

[0034] The rea...

Embodiment 2

[0048]Embodiment 2 The composition of detection kit of the present invention and its using method

[0049] 1. Kit composition

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of a GTF2IRD2 autoantibody detection reagent in preparation of a lung cancer screening kit. It is foundfor the first time that the autoantibody level of the GTF2IRD2 protein in serum of a lung cancer patient is significantly higher than that of a healthy patient. The reagent for detecting the GTF2IRD2protein autoantibody is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of a GTF2IRD2 autoantibody detection reagent in the preparation of a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488
Inventor 张立李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com