The invention discloses a biomarker panel for TA (Takayasu arteritis) detection and an application of the biomarker panel to preparation of reagents for TA diagnosis. The biomarker panel totally contains 22 biomarkers, wherein a first group contains 13 target antigens which are used for differential diagnosis of TA and non-TA by detecting autoantibody level of SPATA7, IL17RB, ZFAND4, PRH2, CLPTM1,PGPEP1, DIXDC1, NDP, SLC27A2, PLA2G16, MAPK1IP1L, HSF2 and NOL3 in serum of a patient, accuracy is 83.6%, and sensitivity and specificity are 50.9% and 94.4% respectively; a second group contains 13target antigens which are used for distinguishing active phase from stationary phase of TA by detecting antibody level of QDPR, PKD1L2, ZFAND4, DIXDC1, NDP, PLA2G16, CIDEA, TRIM36, EIF3H, USF2, AGGF1,SPANXN2 and MCM3 in serum of a patient with TA, accuracy is 96.2%, and sensitivity and specificity are 96.19% and 96.4% respectively.