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Application of ERP27 autoantibody detection reagent to preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no lung cancer, no ERP27 protein, etc., and achieve good application prospects, effective screening, and low patient harm Effect

Active Publication Date: 2019-08-09
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no related reports on ERP27 protein autoantibodies, and there is no prior art related to lung cancer.

Method used

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  • Application of ERP27 autoantibody detection reagent to preparation of lung cancer screening kit
  • Application of ERP27 autoantibody detection reagent to preparation of lung cancer screening kit
  • Application of ERP27 autoantibody detection reagent to preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Relationship between ERP27 autoantibodies in plasma and lung cancer

[0026] 1. Clinical data

[0027] Select 30 cases of lung cancer patients and 30 cases of healthy controls. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 30 30 age 58±10.5 42±8.9 male ratio 20(66.7％) 13(46.7％)

[0029] 2. Detection principle

[0030] HuProt TM ERP27 protein is immobilized on the human protein custom chip. After incubation with serum, ERP27 autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it, and the unbound antibodies and other proteins will be removed by washing. Then use anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) to detect, and read the signal through a fluorescent scanner. The strength of the s...

Embodiment 2

[0044] Embodiment 2 The composition of detection kit of the present invention and its application method

[0045] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, in particular to application of an ERP27 autoantibody detecting reagent to preparation of a lung cancer screening kit. It is found for the first time that the autoantibody level of ERP27 proteins in serum of lung cancer patients is significantly higher than that of healthy patients. The reagent for detecting the autoantibody of the ERP27 proteins is used for preparing the lung cancer screening kit, and lung cancers can be effectively screened.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of ERP27 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/533G01N33/535
CPCG01N33/533G01N33/535G01N33/57423G01N33/57484G01N33/6854G01N2333/4746
Inventor 李为民张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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