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Use of PDE9A autoantibody detection reagent in preparing lung cancer screening kit

An autoantibody and kit technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of no lung cancer, no PDE9A protein, etc., and achieve good application prospects, effective screening, and low patient harm.

Pending Publication Date: 2019-11-05
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on PDE9A protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Use of PDE9A autoantibody detection reagent in preparing lung cancer screening kit
  • Use of PDE9A autoantibody detection reagent in preparing lung cancer screening kit
  • Use of PDE9A autoantibody detection reagent in preparing lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Relationship between PDE9A autoantibodies in plasma and lung cancer

[0026] 1. Clinical data

[0027] 30 cases of lung cancer patients and 30 cases of healthy controls were selected. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 30 30 age 58±10.5 42±8.9 male ratio 20(66.7％) 13(46.7％)

[0029] 2. Detection principle

[0030] HuProt TM PDE9A protein is immobilized on the human protein custom chip. After incubation with serum, PDE9A autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it, and unbound antibodies and other proteins will be removed by washing. Then use anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) to detect, and read the signal through a fluorescent scanner. The strength of th...

Embodiment 2

[0047] Embodiment 2 The composition of detection kit of the present invention and its application method

[0048] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, in particular to the use of a PDE9A autoantibody detection reagent in preparing a lung cancer screening kit. The use of a PDE9A autoantibody detection reagent in preparing a lung cancer screening kit finds that the level of autoantibodies of PDE9A protein in serum of lung cancer patients is significantly higher than that of healthy patients for the first time. The use of a PDE9A autoantibody detection reagent in preparing a lung cancer screening kit uses the reagent for detecting the PDE9A protein autoantibody to prepare a lung cancer screening kit, and can effectively screen the lung cancer.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of PDE9A autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 倪银芸张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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