Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of EEF2K (Eukaryotic Elongation Factor 2 Kinase) autoantibody detection reagent to preparation of lung cancer detection kit

An autoantibody and kit technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of no lung cancer reports, and achieve the effect of good application prospects, low patient harm, and effective screening

Active Publication Date: 2019-12-20
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports about EEF2K protein autoantibodies, and there is no report on EEF2K protein autoantibodies and lung cancer.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of EEF2K (Eukaryotic Elongation Factor 2 Kinase) autoantibody detection reagent to preparation of lung cancer detection kit
  • Application of EEF2K (Eukaryotic Elongation Factor 2 Kinase) autoantibody detection reagent to preparation of lung cancer detection kit
  • Application of EEF2K (Eukaryotic Elongation Factor 2 Kinase) autoantibody detection reagent to preparation of lung cancer detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Relationship between EEF2K autoantibodies in plasma and lung cancer

[0028] 1. Clinical data

[0029] Select 30 cases of lung cancer patients, 29 cases of benign lung diseases (tuberculosis, hamartoma and other non-malignant tumors), and 29 cases of healthy controls. The basic information is as follows:

[0030]

[0031] 2. Detection principle

[0032] HuProt TM EEF2K protein (full-length protein, Ensemble number is ENSP00000456243) is immobilized on the human protein custom chip. After incubation with serum, EEF2K autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it. Wash to remove unbound antibodies and other proteins, then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green), and read the signal through a fluorescent scanner. The strength of the signal is positively correlated with ...

Embodiment 2

[0050] Embodiment 2 The composition of detection kit of the present invention and its using method

[0051] 1. Kit composition

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of in-vitro diagnosis reagents, in particular to application of an EEF2K (Eukaryotic Elongation Factor 2 Kinase) autoantibody detection reagent to the preparation ofa lung cancer screening kit. The invention first discovers that the autoantibody level of EEF2K proteins in the serum of a lung cancer patient is remarkably higher than that of a healthy patient. Thereagent for detecting the EEF2K protein autoantibody is used for preparing the lung cancer screening kit, so that the effective screening of the lung cancer can be realized.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of EEF2K autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 李蕊岑唐怀蓉王婷
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com