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Application of KLC3 autoantibody detection reagent to preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as no KLC3 protein, and achieve good application prospects, effective screening, and low patient harm

Active Publication Date: 2019-11-05
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on KLC3 protein as a cancer marker; there is no report on the relationship between KLC3 protein autoantibodies and lung cancer

Method used

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  • Application of KLC3 autoantibody detection reagent to preparation of lung cancer screening kit
  • Application of KLC3 autoantibody detection reagent to preparation of lung cancer screening kit
  • Application of KLC3 autoantibody detection reagent to preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Relationship between KLC3 autoantibodies in plasma and lung cancer

[0026] 1. Clinical data

[0027] Select 30 cases of lung cancer patients and 30 cases of healthy controls. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 30 30 age 58±10.5 42±8.9 male ratio 20(66.7％) 13(46.7％)

[0029] 2. Detection principle

[0030] HuProt TM The KLC3 protein is immobilized on the human protein custom chip. After incubation with serum, the KLC3 autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it, and the unbound antibodies and other proteins will be removed by washing. Then use anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) to detect, and read the signal through a fluorescent scanner. The strength of ...

Embodiment 2

[0047] Embodiment 2 The composition of detection kit of the present invention and its using method

[0048] 1. Kit composition

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Abstract

The invention relates to the field of in-vitro diagnosis reagents, in particular to application of a KLC3 autoantibody detection reagent to preparation of a lung cancer screening kit. According to theapplication, it is discovered for the first time that the autoantibody level of KLC3 protein in serum of a lung cancer patient is remarkably higher than that of a healthy patient. Through the application, the reagent for detecting a KLC3 protein autoantibody is used for preparing the lung cancer screening kit, and effective screening of a lung cancer can be realized.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of KLC3 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer ...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423
Inventor 刘志强张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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