Establishment Method of Animal Model of Lingnan Damp-Heat Syndrome

A technology of animal model and establishment method, which is applied in the field of establishment of Lingnan damp-heat syndrome animal model, and achieves the effects of simple modeling method, good modeling stability and repeatability, and easy operation and control

Active Publication Date: 2021-11-12
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a method for establishing an animal model of Lingnan damp-heat syndrome in view of the problems existing in the existing techniques for establishing animal damp-heat syndrome

Method used

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  • Establishment Method of Animal Model of Lingnan Damp-Heat Syndrome
  • Establishment Method of Animal Model of Lingnan Damp-Heat Syndrome
  • Establishment Method of Animal Model of Lingnan Damp-Heat Syndrome

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Experimental program
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Effect test

Embodiment 1

[0039] 1 Model preparation materials and methods

[0040] 1.1 Animals: 12 SPF grade BALB / c mice, half male and half male, 3 weeks old, weighing 20-24 g, provided by the Guangdong Provincial Laboratory Animal Center, experimental animal license number: SYXK (Guangdong) 2015-0109, The mice were reared adaptively in the SPF animal room for one week under the conditions of temperature of 22° C., humidity of 55%, and illumination time of 12 h before the experiment was carried out.

[0041] 1.2 SPF grade litter and SPF grade feed

[0042] 1.3 Reagents:

[0043] Glycogen PAS staining solution (Beijing Regen Biotechnology Co., Ltd.)

[0044] Lendrum's Fluorescent Rosé Rye Yellow (Pan's Cell) Staining Kit (Shanghai Gefan Biotechnology Co., Ltd.)

[0045] Anhydrous ethanol (Sinopharm Group Chemical Reagent Co., Ltd.)

[0046] Xylene (Sinopharm Group Chemical Reagent Co., Ltd.)

[0047] EDTA (PH8.0) (Biyuntian)

[0048] PBS buffer (Gibco)

[0049] Antigen retrieval solution

[00...

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Abstract

The invention discloses a method for establishing an animal model of Lingnan damp-heat syndrome. The method includes placing mice in a damp-heat environment for modeling and feeding, and feeding them for 14 to 28 days to obtain an animal model. During the feeding period, the mice are given feed and bedding. Drinking water and bedding are replaced every day. The hot and humid environment conditions are 34°C to 36°C during the day, 85% to 90% humidity for 12 hours, and 18°C ​​to 22°C at night, 55% to 65% humidity for 12 hours. The establishment method of the model is more suitable for the climate, geographical environment and customs and culture of the Lingnan area, and the modeling method is also simpler, with good stability, good repeatability, and easy operation and control.

Description

technical field [0001] The invention relates to animal model experiment technology, in particular to a method for establishing an animal model of Lingnan damp-heat syndrome. Background technique [0002] Damp-heat syndrome is a common TCM syndrome, which is mainly manifested as head and body drowsiness, laziness and less speech, loose stools and other discomforts. In addition to mild discomfort, it can also be seen in many diseases, becoming a syndrome combined with other diseases. According to its etiology, it can be divided into exogenous damp-heat and endogenous damp-heat. Exogenous damp-heat is generally caused by environmental factors, and endogenous damp-heat is generally caused by eating emotions. It is generally believed that the two have interrelated mechanisms and pathways. . In the Lingnan region of southeastern China, due to the humid and hot geographical climate, long sunshine hours, high temperature, and abundant rain, it is easy to cause the ground to wet an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 骆欢欢陈颂郑裕华郑锋玲周祎青王婷谢婧
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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