A kind of construction method and application of fetal-derived bipolar disorder rat model

Abstract

The invention relates to a constructing method of a fetal-original bipolar disorder animal model and application thereof. The fetal-original bipolar disorder animal model is characterized in that a rodent animal is injected with 0.1 to 2.0mg / kg of dexamethasone in a subcutaneous way 9 to 20 days after getting pregnant; the rodent animal is conventionally and normally fed to 10 weeks after birth, the chronic stimulation by ice water swimming is performed for two weeks, and then the typical feature similar to human bipolar disorder appears. The fetal-original bipolar disorder animal model is novel, reliable, simple and convenient, and has important meaning on the instruction of clinical reasonable drug utilization in pregnant period, study of fetal-original bipolar disorder action mechanismand molecular targets, screening of intrauterine environment interference matters and medicines, and the like.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of fetal bipolar disorder. Background technique [0002] Bipolar disorder, also known as bipolar disorder (BD), is a mental disorder characterized by abnormal mania or depression. During a manic episode, it manifests as elevated mood, lack of sleep, increased sexual activity, and increased activity; while during a depressive episode, it manifests as depressed mood, slowed thinking, and decreased activity. In severe cases, psychotic symptoms such as hallucinations, delusions, or catatonic symptoms may appear during the acute phase of the attack. Clinically, it can be distinguished by these characteristic manifestations of the disease [1,2] . According to the classification criteria in Chapter 5 of the Diagnostic and Statistical Manual of Diseases (DSM-5), BD is divided into three types: 1) Bipolar disorder type I, with...

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[0004] However, the existing BD animal models are treated with different intervention factors in adulthood, such as the administration of psychoactive drugs opioid peptides and withdrawal of drugs after treatment, resulting in mice being sensitive to small stimuli, that is, increased irritability; Treatment with amphetamine, a stimulating drug, caused sleep loss in mice; keeping mice in isolation increased their aggressiveness, etc. [12]

Although these modeling methods can simulate the disease phenotype that occurs in BD, the emotional abnormalities of the reaction are all one-way, and the time of administration is after birth rather than during pregnancy, so it can only reflect the environment after birth or the "secondary effect" of drugs. Part of the emotional abnormality caused by "the second blow" is quite different from the pathogenesis of fetal BD

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