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A puffer fish ace inhibitory peptide and its preparation method

A technology of inhibition peptide and inhibition rate, applied in the field of pufferfish ACE inhibition peptide and its preparation, to achieve the effect of improving inhibition effect and high stability

Active Publication Date: 2021-05-25
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are existing technologies for preparing ACE-inhibiting peptides from hazelnuts, yak milk, shellfish, wolfberry and other foods, but there is no technology for preparing ACE-inhibiting peptides from puffer fish, especially puffer fish skin as raw materials

Method used

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  • A puffer fish ace inhibitory peptide and its preparation method
  • A puffer fish ace inhibitory peptide and its preparation method
  • A puffer fish ace inhibitory peptide and its preparation method

Examples

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Embodiment Construction

[0034] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0035] The puffer fish ACE inhibitory peptide provided in this example is a nonapeptide, and its amino acid sequence is: GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg).

[0036] The molecular weight of nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) is 989.5Da.

[0037] This embodiment also provides a preparation method of puffer fish ACE inhibitory peptide, comprising the following steps:

[0038]S1: Preparation of puffer fish enzymatic hydrolyzate, after beating 100g puffer fish skin, add 300mL distilled water and stir to form protein solution, adjust pH value to 8.0 with NaOH, then add alkaline protease, the enzyme amount is 500U / g, Enzyme hydrolyze at 55°C for 10 hours, keep the pH value unchanged, heat at 90°C for 15 minutes after enzymolysis, then cool to room temperature, adjust the pH value to neutral with HCl, then use 5000r / mi...

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Abstract

The invention discloses a puffer fish ACE inhibitory peptide and a preparation method thereof, belonging to the technical field of ACE inhibitory peptides. A method for preparing a puffer fish ACE inhibitory peptide comprises the following steps: S1: preparation of puffer fish enzymatic hydrolyzate, S2: Ultrafiltration separation of pufferfish enzymatic hydrolyzate, S3: separation and purification of polypeptide, S4: sequence identification of polypeptide, S5: synthesis and activity determination of polypeptide. A puffer fish ACE inhibitory peptide, the inhibitory peptide is nonapeptide, and the amino acid sequence is: GDRGFPGER (Gly‑Asp‑Arg‑Gly‑Phe‑Pro‑Gly‑Glu‑Arg). The puffer fish ACE inhibitory peptide and the preparation method thereof of the present invention use puffer fish skin as a raw material to obtain a nonapeptide amino acid sequence, and the prepared polypeptide has ACE inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of ACE inhibitory peptides, and in particular relates to a puffer fish ACE inhibitory peptide and a preparation method thereof. Background technique [0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one. [0003] Pufferfish is a bottom fish in the warm temperate zone and tropical coastal waters. It inhabits the middle and lower layers of the ocean. A few species enter freshwater rivers. When encountering external danger, the whole body floats to the surface in a spherical shape, and at the same time, the small spines on the skin stand up. , for self-defense. [0004] Angiotensin-converting enzyme (ACE) inhibitory peptide is a small ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/16C12P21/06A61P9/12
CPCA61P9/12C07K7/06C12P21/06
Inventor 苏永昌刘智禹刘淑集陈贝乔琨许旻陈晓婷
Owner FISHERIES RES INST OF FUJIAN
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