A method for preparing noni polysaccharide with high anti-cancer and antioxidant activity in a stepped physical field
An anti-oxidant activity and physical field technology, applied in the direction of anti-toxic agents, anti-tumor drugs, drug combinations, etc., can solve the problems of not preparing low-molecular-weight polysaccharides, low yield of noni polysaccharides, long time, etc., to achieve rapid and efficient preparation, Antioxidant performance and anticancer ability improvement, effect of molecular weight reduction
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Embodiment 1
[0034] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:
[0035] Noni fruit raw material processing: dry Noni fruit (Noni fruit is a mature Noni fruit in the white ripe stage) at 50°C, powder, sieve (over 80 mesh sieve), add 4 times the volume percentage of 80% ethanol by volume The solution was refluxed for 4 hours to degrease and decolorize, and then dried to obtain noni fruit powder.
[0036] Cascade physical field-assisted extraction of noni polysaccharides: take 35g of noni fruit powder according to the ratio of material to liquid 1:35 (the mass of noni fruit powder and distilled water in the ratio of material to liquid are in grams and milliliters respectively) and add distilled water into the intermittent pulse electric field extraction equipment The medium was treated 30 times with a pulse frequency of 1 Hz and a treatment field strength of ...
Embodiment 2
[0073] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:
[0074] Noni fruit raw material processing: dry the Noni fruit (the Noni fruit is the mature Noni fruit of the white ripe stage) at 48°C, dry and beat the powder and sieve (over 80 mesh sieve), add 4 times the volume percentage of 85% ethanol solution by volume Reflux treatment for 4 hours to degrease and decolorize, then dry to obtain noni fruit powder.
[0075] Cascade physical field-assisted extraction of noni polysaccharides: Take 30g of noni fruit powder and add distilled water according to the ratio of material to liquid 1:30, and put it into the intermittent pulse electric field extraction equipment for 35 times, the pulse frequency is 5Hz, and the treatment field strength is 5kV / cm, then Ultrasonic equipment was used for ultrasonic treatment for 28 minutes, the ultrasonic power was 600...
Embodiment 3
[0093] Such as figure 1 As shown, a method for preparing noni polysaccharides with high anticancer and antioxidative activity in a cascade physical field comprises the following steps:
[0094] Noni fruit raw material processing: Noni fruit (noni fruit is a ripe noni fruit in the white ripe stage) is dried at 45°C, powdered and sieved (through a 100-mesh sieve), and 4 times of 75% ethanol solution is added by volume to reflux for 4 hours After degreasing and decolorizing, dry the noni fruit powder.
[0095] Cascade physical field-assisted extraction of noni polysaccharides: Take 25g of noni fruit powder and add distilled water according to the ratio of material to liquid 1:25, and put it into the intermittent pulse electric field extraction equipment for 40 times, the pulse frequency is 4Hz, and the treatment field strength is 6kV / cm, then Ultrasonic equipment was used for ultrasonic treatment for 20 minutes, the ultrasonic power was 600W, the ultrasonic frequency was 40kHz, ...
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