A method for sodium urate inducing myeloid suppressor cells
A technology of inhibitory cells and sodium urate, applied in the field of biomedicine, can solve the problems of small number of cells and lack of detailed description of in vitro amplification methods, and achieve the effect of good application prospects.
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Embodiment 1
[0034] Example 1 sodium urate transforms mouse monocytes into myeloid suppressor cells (MDSCs)
[0035] 1. Experimental method
[0036] In this embodiment, a method for inducing myeloid suppressor cells by sodium urate comprises the following steps:
[0037] (1) Isolation of monocytes from mouse bone marrow:
[0038] 1) Kill 6-week-old C57 mice by cervical dislocation, immerse the mice in 75% alcohol for 3 minutes to disinfect; take out the mice, cut the skin on both sides of the mice with ophthalmic scissors, and cut off both sides of the legs from the hip joint bone. Carefully cut off the attached muscles of the femur and tibia, without injuring the articular surface, and avoid exposing the bone marrow cavity;
[0039] 2) In a biological safety cabinet, soak the stripped femur and tibia in 75% alcohol for 3 minutes and transfer them to pre-cooled RPMI 1640 medium;
[0040] 3) Gently cut open both ends of the femur or tibia with ophthalmic scissors, then use a 5ml sterile...
Embodiment 2
[0069] Example 2 Sodium urate converts human peripheral blood mononuclear cells into myeloid suppressor cells (MDSCs)
[0070] 1. Experimental method
[0071] In this embodiment, a method for inducing myeloid suppressor cells by sodium urate comprises the following steps:
[0072] (1) Isolation of mononuclear cells from human peripheral blood:
[0073] Take 5ml of peripheral blood from healthy volunteers and separate mononuclear cells;
[0074] The specific method is as follows:
[0075] 1) Use EDTA-containing anticoagulant blood collection tubes to collect peripheral blood from healthy volunteers, then add 3 times the volume of PBS solution, and mix gently;
[0076] 2) Add 3ml of lymphocyte separation solution to a 50ml sterile centrifuge tube; then use a pipette to slowly add the blood dilution solution to the upper layer of the lymphocyte separation solution along the tube wall, at 18°C, 600g (liter 5, drop 0) , centrifuge for 22min;
[0077] 3) After centrifugation, s...
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