System and method for en masse patterning of molecule structures

A technology of molecules and solutions, applied in the field of stretching nucleic acid molecules, can solve problems such as high entropy cost and difficult molecular loading

Active Publication Date: 2020-06-12
WISCONSIN ALUMNI RES FOUND +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequent studies improved the slit geometry (250nmx 400nm) and ionic strength conditions, which further enhanced DNA stretching ( where L is the molecular profile length), but loading molecules into nanoslits becomes more difficult
This is a common problem affecting most nanofluidic devices due to the high entropic cost when passing a large number of random coil molecules through the slit geometry compared to the DNA persistence length

Method used

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  • System and method for en masse patterning of molecule structures
  • System and method for en masse patterning of molecule structures
  • System and method for en masse patterning of molecule structures

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Embodiment Construction

[0044] All referenced patents, applications, and non-patent literature cited in this disclosure are hereby incorporated by reference in their entirety. In case of inconsistency between the reference and the present disclosure, the present disclosure controls.

[0045] The present disclosure provides devices, systems, and methods described in the following statements, claims, and this specification.

[0046] Whenever reference is made herein to molecules, molecules of interest, nucleic acid molecules or nucleic acid molecules of interest, the present disclosure also contemplates deformable objects such as particles, with or without effective charge, random coil proteins, synthetic polyelectrolytes, dyes quality, synthetic polymers, etc. For uncharged objects, diaphoretic forces can provide the transport described herein, relative to charged objects or molecules and the corresponding forces.

[0047] refer to Figure 1A , shows a microfluidic device 10 according to aspects of th...

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Abstract

Devices, systems, and methods for en masse patterning of nucleic acid molecule structures are disclosed. The devices can include microchannels and nanoslits. The microchannels and nanoslits can be connected by parking chambers. The systems and methods can utilize the geometry of the devices in coordination with a voltage application routine to park nucleic acid molecules in the parking chambers and subsequently inject the nucleic acid molecules into the nanoslits. The methods can be utilized to present nucleic acid molecules in a fashion suitable for genomic analysis. The methods can also be utilized to provide size selection of the nucleic acid molecules.

Description

[0001] Cross References to Related Applications [0002] This application is related to, claims priority to, U.S. Provisional Patent Application No. 62 / 506,992, filed May 16, 2016, which is hereby incorporated by reference for all purposes. [0003] Statement Regarding Federally Funded Research [0004] This invention was made with Government support No. HG000225 from the National Institutes of Health. The government has certain rights in this invention. Background technique [0005] The field of the invention is molecular manipulation. More specifically, the present invention relates to stretching nucleic acid molecules to better visualize certain portions of the nucleic acid molecule for observational studies by various techniques or for isolating various populations of nucleic acid molecules. [0006] The Precision Medicine Initiative is pressing to develop new ways to understand the molecular basis of disease by measuring individuals in detail, which could grow to a col...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N27/447G01N33/487
CPCB01L3/502761G01N27/44791G01N33/48721B01L2200/0663B01L2300/0645B01L2300/0816B01L2300/0896
InventorD·C·施瓦茨K·L·考纳福斯基-谢弗J·P·贺南德斯奥蒂斯K·D·波塔摩西斯K·朱J·J·迪帕波罗T·奥代克
OwnerWISCONSIN ALUMNI RES FOUND