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Rhizopus oryzae ch5 and its application in the extraction of polysaccharide from Zizhizhi

A technology of Rhizopus oryzae and Zizhi powder is applied in the field of bioengineering, which can solve the problems of polysaccharide structure damage and physiological activity reduction, and achieve the effects of improving extraction yield and promoting dissociation.

Active Publication Date: 2022-07-08
深圳立专信息科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some advanced technologies have greatly improved the yield of polysaccharides, some methods will destroy the polysaccharide structure and reduce its physiological activity

Method used

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  • Rhizopus oryzae ch5 and its application in the extraction of polysaccharide from Zizhizhi
  • Rhizopus oryzae ch5 and its application in the extraction of polysaccharide from Zizhizhi
  • Rhizopus oryzae ch5 and its application in the extraction of polysaccharide from Zizhizhi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, screening and identification of fermentation strains

[0023] 1. Isolation and screening of strain CH5

[0024] (1) Add 20 g of purple zhi powder that has been pulverized and passed through a 40-mesh sieve into a 250-mL conical flask, and then add 30 mL of sterile water to moisten, and cultivate at a constant temperature of 28°C for 4 days. Dilute the enriched culture overgrown with sterile water 1 x 10 6 After doubling, it was coated on the PDA plate medium, cultivated at a constant temperature of 28 °C for 3 days, and the mold colonies with different colors and shapes were picked and transferred to fresh PDA medium, and placed at a constant temperature of 28 °C for 3 days to obtain 7 pure cultured strains, respectively. Numbered CH1–CH7. Add 5 mL of sterile water to the fresh plate culture of the 7 strains, stir with an inoculating loop to suspend the spores, transfer the spore liquid to a sterile test tube, and use sterile water to adjust the sp...

Embodiment 2

[0037] Embodiment 2 Rhizopus oryzae CH5 is applied to the extraction of Zizhi polysaccharide

[0038] (1) Preparation of Rhizopus oryzae CH5 spore liquid: The Rhizopus oryzae CH5 plate colony stored in a refrigerator at 4°C was inoculated into a fresh PDA plate medium, cultured at a constant temperature of 28°C for 3 days, and 5 mL of sterile water was added to the plate culture. Stir with an inoculating loop to suspend the spores, transfer the spore solution to a sterile test tube, and adjust the spore concentration to 4 × 10 with sterile water. 8 each / mL; Described PDA plate medium composition and preparation method are with embodiment 1;

[0039] (2) Sterilize a 500-mL conical flask with dry heat at 160°C for 2 hours, add 20 g of purple zhi powder that has been pulverized and passed through a 40-mesh sieve, add 30 mL of sterile water, and then inoculate 2 mL of Step (1) to prepare a suspension of Rhizopus oryzae CH5 spores liquid, mix well. The triangular flask was tied w...

Embodiment 3

[0044] Embodiment 3 Rhizopus oryzae CH5 is applied to the extraction of Zizhi polysaccharide

[0045] (1) Preparation of Rhizopus oryzae CH5 spore liquid: cryopreserved Rhizopus oryzae CH5 (freeze-dried tube or plate colony) was inoculated into fresh PDA plate medium, incubated at 28°C for 3d, and 5mL of sterile water was added to the plate In the culture, the spores were suspended by stirring with an inoculating loop, and the spore solution was transferred to a sterile test tube, and the spore concentration was adjusted to 5 × 10 with sterile water. 8 each / mL; Described PDA plate medium composition and preparation method are with embodiment 1;

[0046] (2) Sterilize a 2-L conical flask by dry heat at 160°C for 2h, add 50g of 40-mesh purple zhi powder, add 100mL of sterile water, and then inoculate 5mL of it. Step (1) Prepare Rhizopus oryzae CH5 spore suspension liquid, mix well. The triangular flask was tied with eight layers of gauze, and after culturing at 30°C for 2 days...

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Abstract

The invention discloses a kind of Rhizopus oryzae CH5 and its application in extracting polysaccharides from Zizhi zhi. The application method is as follows: inoculating the spores of Rhizopus oryzae CH5 in Zizhi powder containing sterile water, and fermenting at 28-30℃ 2-3d, after the fermented product is dried, fermented purple zhi powder is obtained; the fermented purple zhi powder is decolorized to obtain decolorized purple zhi powder; the decolorized purple zhi powder is extracted with water and concentrated to obtain a concentrated liquid; the concentrated liquid is subjected to alcohol precipitation and vacuum drying to obtain purple zhi powder Crude polysaccharides. Rhizopus oryzae CH5 is an excellent strain isolated from the microbial enrichment culture of Zizhi, and obtained through screening. It grows moderately in Zizhi powder to produce polysaccharide hydrolase, which can hydrolyze the structure of insoluble polysaccharide in Zizhi and promote the decomposition of bound soluble polysaccharide. separation, thereby significantly improving the extraction yield of Zizhi polysaccharide. Under the optimal process conditions, the extraction yield of Zizhi polysaccharide was 5.92%, which was more than 35% higher than that of the unfermented extraction method.

Description

(1) Technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for improving the extraction yield of Zizhi polysaccharide by adopting a microbial fermentation method. (2) Background technology [0002] Ganoderma sinense, also known as "Chinese Ganoderma lucidum" and "Xiancao", also known as Heizhi and Xuanzhi, is a fungus of the basidiomycete Polyporaceae Ganoderma genus. Zizhi is a kind of precious traditional Chinese medicine Ganoderma lucidum in our country. It is recorded in the Compendium of Materia Medica: Zizhi is a Muzhi, with a sweet, warm and non-toxic smell; it is mainly used for deafness, benefiting joints, protecting the mind, nourishing essence and qi, strengthening muscles and bones, and good color. Take it for a long time, keep your body light and prolong your life. Ganoderma lucidum is included in the 2015 edition of Chinese Pharmacopoeia, one of the Ganoderma lucidum. It has the functions o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C08B37/00C12R1/845
CPCC12P19/04C08B37/0003C12R2001/845C12N1/145
Inventor 陈虹张建芬雷超谢广发
Owner 深圳立专信息科技有限公司
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