Rhizopus oryzae ch5 and its application in the extraction of polysaccharide from Zizhizhi

Abstract

The invention discloses a kind of Rhizopus oryzae CH5 and its application in extracting polysaccharides from Zizhi zhi. The application method is as follows: inoculating the spores of Rhizopus oryzae CH5 in Zizhi powder containing sterile water, and fermenting at 28-30℃ 2-3d, after the fermented product is dried, fermented purple zhi powder is obtained; the fermented purple zhi powder is decolorized to obtain decolorized purple zhi powder; the decolorized purple zhi powder is extracted with water and concentrated to obtain a concentrated liquid; the concentrated liquid is subjected to alcohol precipitation and vacuum drying to obtain purple zhi powder Crude polysaccharides. Rhizopus oryzae CH5 is an excellent strain isolated from the microbial enrichment culture of Zizhi, and obtained through screening. It grows moderately in Zizhi powder to produce polysaccharide hydrolase, which can hydrolyze the structure of insoluble polysaccharide in Zizhi and promote the decomposition of bound soluble polysaccharide. separation, thereby significantly improving the extraction yield of Zizhi polysaccharide. Under the optimal process conditions, the extraction yield of Zizhi polysaccharide was 5.92%, which was more than 35% higher than that of the unfermented extraction method.

Description

(1) Technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for improving the extraction yield of Zizhi polysaccharide by adopting a microbial fermentation method. (2) Background technology

[0002] Ganoderma sinense, also known as "Chinese Ganoderma lucidum" and "Xiancao", also known as Heizhi and Xuanzhi, is a fungus of the basidiomycete Polyporaceae Ganoderma genus. Zizhi is a kind of precious traditional Chinese medicine Ganoderma lucidum in our country. It is recorded in the Compendium of Materia Medica: Zizhi is a Muzhi, with a sweet, warm and non-toxic smell; it is mainly used for deafness, benefiting joints, protecting the mind, nourishing essence and qi, strengthening muscles and bones, and good color. Take it for a long time, keep your body light and prolong your life. Ganoderma lucidum is included in the 2015 edition of Chinese Pharmacopoeia, one of the Ganoderma lucidum. It has the functions o...

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