Pharmaceutical composition for chemotherapy and immunotherapy combined treatment

Abstract

The invention discloses a pharmaceutical composition for chemotherapy and immunotherapy combined treatment. The pharmaceutical composition is prepared from: a first component alginate and a second component chemotherapeutic drug; the alginate can form a porous gel with calcium ions in a body, and the alginate is one or more of sodium alginate, potassium alginate and ammonium alginate; and the second component chemotherapeutic drug can cause immungentic cell death. The pharmaceutical composition belongs to an efficient tumor-specific immunotherapy program, the tumor in situ can be effectively killed, and the growth of distant metastatic tumors and the recurrence probability of tumor can be suppressed and reduced through immune response.

Description

technical field

[0001] The invention relates to a pharmaceutical composition for combination therapy of chemotherapy and immunity, as well as a preparation method and application. Background technique

[0002] The human immune system is a defense network covering the whole body. The first line of defense to protect the body is: skin, mucous membranes and their secretions, cell membranes, respiratory tract, gastrointestinal tract, urethra and kidneys; the second line of defense is: phagocytosis, antibacterial proteins and inflammatory response; the third line of defense is mainly composed of immune organs (tonsils, lymph nodes, thymus, bone marrow, and spleen, etc.) and immune cells (lymphocytes, phagocytes, etc.) with the help of blood circulation and lymphatic circulation. The main functions of the immune organs and immune cells are as follows: 1. Protection: resist the invasion of antigens, prevent the occurrence of diseases, and maintain the health of the human body; 2. C...

Examples

Embodiment 1

[0101] Example 1: Preparation and use of sodium alginate (the first type of component) and imiquimod (the third type of component) hydrochloride composition freeze-dried powder injection

[0102] Step 1: Preparation of imiquimod (the third component) hydrochloride. Weigh 50-100 mg of imiquimod into a 50 ml glass mixing container, add 1 ml of 1M dilute hydrochloric acid therein, and add deionized water to dilute after the white powdery imiquimod is fully dissolved until it is colorless and transparent, so that The final concentration of imiquimod is 2.5-5 mg / ml. The solution was freeze-dried to obtain freeze-dried powder of imiquimod hydrochloride. The purpose of this step is to convert the water-insoluble imiquimod into the water-soluble hydrochloride form. Sufficient lyophilization time is required to ensure complete removal of hydrochloric acid residues.

[0103] Step 2: The following three methods can be used to prepare the freeze-dried powder injection of the hydrochlor...

Embodiment 2

[0117] Example 2: Sodium alginate (the first type of component) and CpG oligonucleotide (the third type of component) composition freeze-dried powder injection

[0118] Step 1: Preparation of sodium alginate and CpG oligonucleotide composition freeze-dried powder injection

[0119] Weighing 10-80 mg of sodium alginate and 0.1-5 mg of CpG oligonucleotides are dissolved in 1 ml of aqueous phase solution, fully shaken until the solution is clear and transparent, and then freeze-drying the solution to obtain a freeze-dried powder injection of the composition.

[0120] Figure 5 It is a scanning electron microscope picture of the freeze-dried powder injection of the composition after reconstitution into a gel. It can be seen from the figure that the composition still has good gelation ability after freeze-drying and reconstitution, and from the electron microscope pictures, it can be seen that there are many micron-scale pores after gelation, which is of great help to the sustaine...

Embodiment 3

[0129] Embodiment three: Sodium alginate (the first type of component) and doxorubicin hydrochloride (the second type of component) composition freeze-dried powder injection

[0130] Step 1: Preparation of freeze-dried powder injection of sodium alginate and doxorubicin hydrochloride composition:

[0131] Method 1: Weigh 20-80 mg of sodium alginate and 0.1-10 mg of doxorubicin hydrochloride and dissolve them in 1 ml of aqueous phase solution, stir with a stirring paddle at a speed of 50-300 rpm until the solution is clear and transparent, and then dissolve The solution is freeze-dried to obtain a freeze-dried powder injection of the composition.

[0132] Method 2: Dissolve 0.1-10 mg of doxorubicin hydrochloride in 1 ml of aqueous phase solution, stir with a stirring paddle at a speed of 50-300 rpm until the solution is clear and transparent, and then dissolve 10-80 mg of sodium alginate Into the aqueous phase solution, add the constantly stirring doxorubicin hydrochloride sol...

technical field

[0001] The invention relates to a pharmaceutical composition for combination therapy of chemotherapy and immunity, as well as a preparation method and application. Background technique

[0002] The human immune system is a defense network covering the whole body. The first line of defense to protect the body is: skin, mucous membranes and their secretions, cell membranes, respiratory tract, gastrointestinal tract, urethra and kidneys; the second line of defense is: phagocytosis, antibacterial proteins and inflammatory response; the third line of defense is mainly composed of immune organs (tonsils, lymph nodes, thymus, bone marrow, and spleen, etc.) and immune cells (lymphocytes, phagocytes, etc.) with the help of blood circulation and lymphatic circulation. The main functions of the immune organs and immune cells are as follows: 1. Protection: resist the invasion of antigens, prevent the occurrence of diseases, and maintain the health of the human body; 2. C...

Examples

Embodiment 1

[0101] Example 1: Preparation and use of sodium alginate (the first type of component) and imiquimod (the third type of component) hydrochloride composition freeze-dried powder injection

[0102] Step 1: Preparation of imiquimod (the third component) hydrochloride. Weigh 50-100 mg of imiquimod into a 50 ml glass mixing container, add 1 ml of 1M dilute hydrochloric acid therein, and add deionized water to dilute after the white powdery imiquimod is fully dissolved until it is colorless and transparent, so that The final concentration of imiquimod is 2.5-5 mg / ml. The solution was freeze-dried to obtain freeze-dried powder of imiquimod hydrochloride. The purpose of this step is to convert the water-insoluble imiquimod into the water-soluble hydrochloride form. Sufficient lyophilization time is required to ensure complete removal of hydrochloric acid residues.

[0103] Step 2: The following three methods can be used to prepare the freeze-dried powder injection of the hydrochlor...

Embodiment 2

[0117] Example 2: Sodium alginate (the first type of component) and CpG oligonucleotide (the third type of component) composition freeze-dried powder injection

[0118] Step 1: Preparation of sodium alginate and CpG oligonucleotide composition freeze-dried powder injection

[0119] Weighing 10-80 mg of sodium alginate and 0.1-5 mg of CpG oligonucleotides are dissolved in 1 ml of aqueous phase solution, fully shaken until the solution is clear and transparent, and then freeze-drying the solution to obtain a freeze-dried powder injection of the composition.

[0120] Figure 5 It is a scanning electron microscope picture of the freeze-dried powder injection of the composition after reconstitution into a gel. It can be seen from the figure that the composition still has good gelation ability after freeze-drying and reconstitution, and from the electron microscope pictures, it can be seen that there are many micron-scale pores after gelation, which is of great help to the sustaine...

Embodiment 3

[0129] Embodiment three: Sodium alginate (the first type of component) and doxorubicin hydrochloride (the second type of component) composition freeze-dried powder injection

[0130] Step 1: Preparation of freeze-dried powder injection of sodium alginate and doxorubicin hydrochloride composition:

[0131] Method 1: Weigh 20-80 mg of sodium alginate and 0.1-10 mg of doxorubicin hydrochloride and dissolve them in 1 ml of aqueous phase solution, stir with a stirring paddle at a speed of 50-300 rpm until the solution is clear and transparent, and then dissolve The solution is freeze-dried to obtain a freeze-dried powder injection of the composition.

[0132] Method 2: Dissolve 0.1-10 mg of doxorubicin hydrochloride in 1 ml of aqueous phase solution, stir with a stirring paddle at a speed of 50-300 rpm until the solution is clear and transparent, and then dissolve 10-80 mg of sodium alginate Into the aqueous phase solution, add the constantly stirring doxorubicin hydrochloride sol...

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