Method for centralized spermatogenesis of male haliotis fulgens of hybrid filial generation of haliotis fulgens

Abstract

The invention discloses a method for centralized spermatogenesis of male haliotis fulgens of a first filial generation of haliotis fulgens. The method comprises the following steps: (1) placing a spermatogenesis box into a water groove, wherein the spermatogenesis box comprises a plurality of independent small chambers, and a through hole is arranged on the wall of each small chamber, and placingone shade-dry stimulated male haliotis fulgens of the first filial generation of the haliotis fulgens into each small chamber; (2) putting disinfected seawater into the water groove until the spermatogenesis box is submerged, and continuously inflating air into the water groove; (3) putting an ovum-free egg liquid of female haliotis discus hannai into the water groove, stirring a water body by inflating, and allowing the ovum-free egg liquid of the female haliotis discus hannai to stimulate the male haliotis fulgens to produce sperms; and (4) after any one male haliotis fulgens of the first filial generation of the haliotis fulgens produces sperms, and allowing the sperms to flow to the small chambers through the through holes arranged on the walls of the small cambers to stimulate the male haliotis fulgens to produce sperms quickly. The method provided by the invention solves the problem of slow and decentralized spermatogenesis of the male haliotis fulgens of the first filial generation of the haliotis fulgens in the conventional hybridization process, improves the spermatogenesis rate of the male haliotis fulgens of the first filial generation of the haliotis fulgens, and guarantees large-scale production effect of hybrid haliotis fulgens juveniles.

Description

technical field [0001] The invention relates to the technical field of abalone production, in particular to a method for intensively producing green abalone hybrid male abalone first-generation male abalone. Background technique [0002] Green abalone, native to the subtropical Pacific coast of America, is a large abalone with high economic value; wrinkled plate abalone is the main abalone species cultivated in northern my country. Green abalone and wrinkled plate abalone are complementary in temperature tolerance and other traits, so the green abalone male and the wrinkled plate abalone currently cultivated in my country can be crossed to breed fast growth, high temperature resistance, and strong stress resistance. This is of great significance for improving the structure of abalone aquaculture species in my country and promoting the development of the entire abalone aquaculture industry chain. [0003] However, due to the high cost of introducing green abalone, the first g...

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Problems solved by technology

[0004] In view of the above-mentioned prior art, the object of the present invention is to provide a method for concentrating the first-generation male abalone hybrid offspring of green abalone on concentrated sperm production, and solve the problem that the first-generation male abalone hybrid offspring of green abalone has relatively slow sperm production in the current hybridization process. And the problem of non-concentration improves the sperm yield of the first generation male abalone hybrids of green abalone and ensures the large-scale production effect of hybrid seedlings

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