A method for embryogenic callus induction and plant regeneration
An embryogenic callus and callus induction technology, applied in the field of plant tissue culture, can solve the problems of low callus induction rate, difficult to repeat differentiation and regeneration, etc.
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[0052] (1) The tubers of Wenyujin are naturally placed in the laboratory at a room temperature of about 25 degrees. It has been observed that May, June and July are the seasons when the tubers germinate the most.
[0053] Cut the sprouts with a length of about 1.5cm with a scalpel, and pay attention to some tuber tissue when cutting, which is more conducive to the inoculation and survival of the sprouts later.
[0054] (2) Rinse the cut buds above with tap water first, remove the soil and dirt on the top, and then place them in a 50ml sterile centrifuge tube, shake them twice with sterilized distilled water on a horizontal shaker, and then use 0.1 The sterile distilled water of g / L Iperilin was shaken for 2 times, and the third time was shaken overnight; the next day, it was washed with sterile distilled water 3 times, and then with 50% plant tissue culture bacteriostatic agent PPM (v / v ) in sterile water for 10min, washed with sterile distilled water for 3 times; then sterili...
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