A method for embryogenic callus induction and plant regeneration

An embryogenic callus and callus induction technology, applied in the field of plant tissue culture, can solve the problems of low callus induction rate, difficult to repeat differentiation and regeneration, etc.

Active Publication Date: 2022-03-25
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wang Xiaohui from Wenzhou Medical College reported the system of Wen Yujin callus induction, differentiation and regeneration, but the callus induction rate of this system is low, and the differentiation and regeneration are difficult to repeat

Method used

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  • A method for embryogenic callus induction and plant regeneration
  • A method for embryogenic callus induction and plant regeneration
  • A method for embryogenic callus induction and plant regeneration

Examples

Experimental program
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Embodiment 1

[0052] (1) The tubers of Wenyujin are naturally placed in the laboratory at a room temperature of about 25 degrees. It has been observed that May, June and July are the seasons when the tubers germinate the most.

[0053] Cut the sprouts with a length of about 1.5cm with a scalpel, and pay attention to some tuber tissue when cutting, which is more conducive to the inoculation and survival of the sprouts later.

[0054] (2) Rinse the cut buds above with tap water first, remove the soil and dirt on the top, and then place them in a 50ml sterile centrifuge tube, shake them twice with sterilized distilled water on a horizontal shaker, and then use 0.1 The sterile distilled water of g / L Iperilin was shaken for 2 times, and the third time was shaken overnight; the next day, it was washed with sterile distilled water 3 times, and then with 50% plant tissue culture bacteriostatic agent PPM (v / v ) in sterile water for 10min, washed with sterile distilled water for 3 times; then sterili...

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Abstract

The invention discloses a method for inducing embryogenic callus and plant regeneration of S. chinensis, and belongs to the technical field of plant tissue culture. The method comprises: (1) taking the young buds on the tubers of Radix Radix et Rhizoma, rinsing them with sterile water after disinfection, and then inoculating them in a young bud culture medium to obtain sterile seedlings of Radix Radix et Rhizoma; (2) transferring the sterile seedlings to the clumps of buds Cultivate in an induction medium to obtain aseptic clump seedlings; (3) take the root bases of the aseptic clump seedlings as explants, inoculate them in the callus induction medium, and cultivate in dark to obtain embryogenic callus of T. vulgaris; (4) ) Inoculate the embryogenic callus of Radix Radix et Rhizoma in a differentiation medium for culture to obtain a regenerated plant of Radix Radix et Rhizoma. The invention adopts multi-step sterilization for the young shoots of Radix chinensis to achieve rapid and effective detoxification; embryogenic callus in good condition is a key step for differentiation and regeneration, and the callus induction medium provided by the invention induces the generation of embryogenic callus The rate reaches about 95%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing embryogenic callus and plant regeneration of S. chinensis. Background technique [0002] Wenyujin (Wen Curcuma) is a perennial herb of the ginger family, turmeric, with a long history of medicinal use. According to Tang's "Medicinal Properties" and Li Shizhen's "Compendium of Materia Medica": Curcuma has the effect of promoting qi, breaking blood, eliminating accumulation and relieving pain. It is called the medicine in blood. In my country's modern medical research and clinical application, Curcuma curcuma is an effective anti-cancer Chinese medicine, and its main components are volatile oil, curcumin and polysaccharides. It has good curative effect in the prevention and treatment of malignant tumor, especially early cervical cancer. The volatile oils are mainly sesquiterpenoids, which are secondary metabolites of plants. Curcuma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 谢恬马孝霞魏昕刘雨恒卫秋慧殷晓浦谌容
Owner HANGZHOU NORMAL UNIVERSITY
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