Animal model construction method for transplanted vein restenosis research

An animal model and construction method technology, applied in animal husbandry and other directions, can solve the problems of unavoidable immune rejection, unfavorable popularization, influence and so on

Inactive Publication Date: 2020-09-11
南昌大学第一附属医院
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the lack of reagents (such as antibodies) for rabbits, the lack of data in the gene bank, and the slow progress of related research will affect the continuity of animal model research and cause inconvenience
Most of the current animal models use the animal's own veins to access the arterial environment through microsuture surgery, which requires the experimenters to have skilled microsurgical techniques, which is not conducive to the popularization an

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  • Animal model construction method for transplanted vein restenosis research
  • Animal model construction method for transplanted vein restenosis research
  • Animal model construction method for transplanted vein restenosis research

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Embodiment Construction

[0020] The specific implementation manners of the present invention will be further described below in conjunction with the drawings and examples. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.

[0021] 1. Animal model construction method and related index detection

[0022] 1.1 Materials

[0023] 1.1.1 Experimental animals

[0024] Eighteen severe immunodeficiency F344RG rats, weighing 150-180g, for 6-8 weeks, with an average arterial pressure of 127±3.4mmhg, were purchased from Beijing Weitongda Biotechnology Co., Ltd. The feeding in the IVC room, the feeding and operation of experimental animals were all completed in the Experimental Animal Center of the Medical Department of Nanchang University. This experiment was approved by the Ethics Committee of the First Affiliated Hospital of Nanchang University.

[0025] 1.1.2 Grafts

[0026] 24 seg...

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Abstract

The invention discloses an animal model construction method for transplanted vein restenosis research, which comprises the following steps of: selecting an F344RG rat with severe immunodeficiency caused by utilizing a CRISPR/Cas gene editing technology at an embryonic stage as an host, and taking the rest great saphenous vein blood vessels of a CABG postoperative patient as a graft; implanting human saphenous veins into the abdominal aorta of F344RG rats using a cannulation method to establish a humanized rat model. The establishment method of the model is simple, convenient and repeatable, the rat death rate is low, operation can be completed by only one person, and a feasible animal model scheme is provided for research on the aspect of CABG postoperative venous bridge restenosis.

Description

technical field [0001] The invention relates to a method for constructing an animal model for the study of grafted vein restenosis. Background technique [0002] With the improvement of people's living standards, coronary atherosclerotic heart disease (CHD, Coronary heart disease) has become the "number one killer" of modern people, and more and more attention has been paid to it. Clinically, in the surgical treatment of CHD, the patient's own great saphenous vein is usually selected for coronary artery bypass grafting (CABG, Coronary artery bypass grafting), but the problem of restenosis of the grafted vein after surgery is difficult to solve. [0003] During the animal validation phase of research on restenosis after grafted veins, it is often necessary to establish animal models of great saphenous vein grafts. At present, rabbits are often used as experimental individuals, and the carotid artery and vein are connected to establish a model. However, the lack of reagents ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K67/0275A01K2207/30A01K2217/054A01K2227/105A01K2267/0375
Inventor 王群
Owner 南昌大学第一附属医院
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