Animal model construction method for transplanted vein restenosis research

Abstract

The invention discloses an animal model construction method for transplanted vein restenosis research, which comprises the following steps of: selecting an F344RG rat with severe immunodeficiency caused by utilizing a CRISPR / Cas gene editing technology at an embryonic stage as an host, and taking the rest great saphenous vein blood vessels of a CABG postoperative patient as a graft; implanting human saphenous veins into the abdominal aorta of F344RG rats using a cannulation method to establish a humanized rat model. The establishment method of the model is simple, convenient and repeatable, the rat death rate is low, operation can be completed by only one person, and a feasible animal model scheme is provided for research on the aspect of CABG postoperative venous bridge restenosis.

Description

technical field [0001] The invention relates to a method for constructing an animal model for the study of grafted vein restenosis. Background technique [0002] With the improvement of people's living standards, coronary atherosclerotic heart disease (CHD, Coronary heart disease) has become the "number one killer" of modern people, and more and more attention has been paid to it. Clinically, in the surgical treatment of CHD, the patient's own great saphenous vein is usually selected for coronary artery bypass grafting (CABG, Coronary artery bypass grafting), but the problem of restenosis of the grafted vein after surgery is difficult to solve. [0003] During the animal validation phase of research on restenosis after grafted veins, it is often necessary to establish animal models of great saphenous vein grafts. At present, rabbits are often used as experimental individuals, and the carotid artery and vein are connected to establish a model. However, the lack of reagents ...

AI Technical Summary

Problems solved by technology

However, the lack of reagents (such as antibodies) for rabbits, the lack of data in the gene bank, and the slow progress of related research will affect the continuity of animal model research and cause inconvenience

Most of the current animal models use the animal's own veins to access the arterial environment through microsuture surgery, which requires the experimenters to have skilled microsurgical techniques, which is not conducive to the popularization and promotion of this research

And the transplanted vein is the autologous vein of the experimental animal instead of the great saphenous vein of the human being. The experimental results can only be used as an analogy reference and no definite conclusion can be drawn.

If the general experimental animal vein is replaced by the human great saphenous vein, it is difficult to avoid the immune rejection caused by species differences affecting the experimental results

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