Constant-temperature device for cell tissue culture

Abstract

The invention discloses a constant temperature device for cell tissue culture. When the constant temperature device for cell tissue culture is used, when a sealing door is opened, a sliding plate loses the acting force of an ejector rod, and the sliding plate slides under the action of a reset spring so as to touch a travel switch; the action of the travel switch triggers the electromagnetic reversing valve to reverse, so that the air flow is discharged from the other outlet of the electromagnetic reversing valve and enters the second air guide pipe, the air flow is discharged from the air outlet of the second air guide pipe, and the air flow is blown in the direction towards the sealing door; therefore, external air is prevented from entering the constant-temperature device when the sealing door is opened, external bacteria are prevented from entering the constant-temperature device and polluting plant cells, and normal cultivation of the plant cells is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of cell culture; in particular, it relates to a constant temperature device for cell tissue culture. Background technique [0002] In plant cell culture, under in vitro conditions, callus or other easily dispersible tissues are placed in a liquid medium for shaking culture to obtain dispersed and free suspension cells, and the cells are multiplied by subculture to obtain a large number of cells A technique for groups. [0003] Plant cell culture mainly includes several steps such as isolation of single cells, cell culture and cell preservation. The methods for separating single cells mainly include physical methods, chemical methods and enzymatic methods. The physical method is to put the loose callus into the liquid medium, and the shaker is continuously vibrated to disperse the cells; the chemical method is to add oxalate (calcium that can bind pectin calcium in the intercellular substance) to the cell s...

AI Technical Summary

Problems solved by technology

[0007] Under normal circumstances, cell culture needs to be carried out under sterile conditions, and sterile air is also introduced into the constant temperature device, but in the process of opening and closing the seals, more or less outside air is also introduced, thus affecting the constant temperature The sterile environment inside the device, which affects the cultivation of plant cells

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