Medical vivisection testing method for Kunming (KM) mouse

Abstract

The invention discloses a medical vivisection testing method for a Kunming (KM) mouse, and relates to the technical field of life science. S3, dissecting: a scalpel is utilized to remove calvaria skinbetween both eyes in a direction perpendicular to the nasal bone of the KM mouse, and meanwhile, skin from the parietal region to the occipital bone between both ears is removed to expose skulls, bone forceps are utilized to enter the skulls for certain depth and cut into the head, then, scissors are utilized to cut open the parietal bone, and the skulls are opened; and S4, sampling: the skulls at two sides outside the cerebrum are separated and taken away to expose complete brain tissue, and surgical scissors and ophthalmic forceps are utilized to peel off meninges. According to the medicalvivisection testing method for the KM mouse, the skulls of the KM mouse can be rapidly separated to obtain complete and pollution-free brain tissue samples including the cerebrum, epencephalon, hypophysis, hypothalamus and the like; compared with a traditional method, the medical vivisection testing method improves the collection integrity of all the tissue samples of the brain, and lowers the risk of operation pollution and sample damage; and the medical vivisection testing method is simple and has a good using effect.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to a medical KM mouse vivisection test method. Background technique [0002] KM mice are the outbred mice with the largest production and usage in my country. The gene pool is large and the gene heterozygosity rate is high. Chinese scholars have successively bred many inbred strains of mice from the outbred group of KM mice. After more than 50 years of selective breeding, the tumor spontaneous rate in KM mice is extremely low. There are certain differences in the growth, development and reproductive performance of closed groups of Kunming mice raised in different places. But their common characteristics are strong disease resistance and adaptability, high reproduction rate and survival rate. The price is cheap, and it is about 70% of the total amount of mice used in my country's biomedical animal experiments. [0003] The existing method for collecting brain tissue samples...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a kind of medical KM mouse vivisection test method, which solves the collection method of the existing KM mouse brain tissue sampl

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