Wnt protein\zif-8 nanocomplex, its preparation method and application

A nanocomposite, ZIF-8 technology, applied in the direction of peptide/protein components, drug combinations, pharmaceutical formulations, etc., can solve the problems of ZIF-8 disintegration speed, strong cytotoxicity, etc. Live, enhance the effect of stability

Active Publication Date: 2021-05-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the zeolitic imidazolate framework-8 (ZIF-8) with zinc (Zn) as the coordination center is an important member of the MOFs family and also has excellent drug-loading properties, but ZIF-8 in the prior art Excessive release of zinc ions has strong cytotoxicity, and the disintegration speed of ZIF-8 is fast, so the concentration of ZIF-8 is strictly limited

Method used

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  • Wnt protein\zif-8 nanocomplex, its preparation method and application
  • Wnt protein\zif-8 nanocomplex, its preparation method and application
  • Wnt protein\zif-8 nanocomplex, its preparation method and application

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preparation example Construction

[0048] The embodiment of the present invention also provides a preparation method of Wnt protein\ZIF-8 nanocomplex, including: Wnt protein\ZIF-8 nanocomplex is synthesized in situ so that Wnt protein is loaded on the ZIF-8 zeolite imidazole ester skeleton.

[0049] Firstly, preparing an imidazole ligand solution: dissolving imidazole materials to form a raw solution containing imidazole materials, and then mixing the original solution with Wnt protein raw materials to form an imidazole ligand solution containing Wnt protein.

[0050] Further, the Wnt protein raw material includes Wnt protein; preferably, the Wnt protein raw material also includes a protective protein; preferably, the protective protein is albumin, preferably BSA; preferably, the Wnt protein raw material is Wnt protein and A mixture formed by the protective protein; the preparation of the mixture includes: mixing Wnt protein and BSA according to the mass ratio of 1:1-1:9.

[0051] That is to say, the Wnt prote...

Embodiment 1

[0061] The present embodiment provides a preparation method of Wnt protein\ZIF-8 nanocomplex, comprising:

[0062] (1) Dissolve 259mg (3.15mmol) of 2-methylimidazole in 0.9mL of enzyme-free water, stir well to dissolve (temperature 30°C, 400rpm), and obtain solution A;

[0063] (2) Dissolve 250 micrograms of Wnt3a in 250 micrograms of BSA solution to form a Wnt3a / BSA mixture;

[0064] (3) Add 500 micrograms of Wnt3a / BSA mixture to solution A and stir thoroughly for 10 minutes (30°C, 400rpm) to obtain solution B;

[0065] (4) Dissolve 13.4mg (0.045mmol) of zinc nitrate hexahydrate in 0.1mL of enzyme-free water, stir and dissolve fully (30°C, 400rpm) to obtain solution C;

[0066] (5) Add solution C to solution B and stir thoroughly for 10 minutes (30°C, 400rpm) to obtain white suspension D;

[0067] (6) Centrifuge the suspension D for 20min (4000rpm), discard the supernatant to obtain the precipitate E; add 1mL of absolute ethanol to the precipitate E, resuspend, centrifuge f...

Embodiment 2- Embodiment 5

[0069] Embodiment 2-Example 5 provides a preparation method of Wnt protein\ZIF-8 nanocomplex and the preparation method of Wnt protein\ZIF-8 nanocomplex provided in Example 1 is basically the same operation, the difference lies in the raw materials and specific Operating conditions vary as follows:

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Abstract

The present invention relates to the technical field of medicine preparation, in particular, to Wnt protein\ZIF-8 nanocomplex, its preparation method and its application. The nanocomposite includes a ZIF-8 zeolite imidazolate framework loaded with a Wnt protein. In the present invention, by wrapping the Wnt protein with ZIF-8, the disintegration rate of ZIF-8 can be effectively slowed down, the release rate of zinc ions is slowed down, and the dosage of ZIF-8 can be increased to a maximum. At the same time, ZIF‑8 encapsulates Wnt protein, which improves the stability of Wnt protein, making it less likely to be inactivated in the body fluid environment or in the process of preparation and transportation, and improving physiological activity. At the same time, the combined use of ZIF-8 and Wnt protein has a good bone-promoting effect, which expands the application range of Wnt protein\ZIF-8 nanocomplex.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to Wnt protein\ZIF-8 nanometer complex, its preparation method and its application. Background technique [0002] In the prior art, protein is often used to treat diseases, but there are many disadvantages in using protein alone. (1) Protein is easily hydrolyzed in body fluid environment; (3) It is difficult for a single protein to directly enter the cell, which in turn affects the physiological and biochemical properties of the protein. Therefore, in order to ensure the physiological and biochemical properties of the protein, it is often necessary to use a carrier. At the same time, even if the carrier is used to load the protein, the protein still has problems such as easy inactivation or low activity. Metal-organic frameworks (MOFs) materials can be used as excellent carriers because of their extremely high specific surface area, high porosity, and structural divers...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K47/22A61K47/42A61K38/17A61P19/08
CPCA61K9/5123A61K9/5169A61K38/1709A61P19/08
Inventor 裴锡波陈崧王剑万乾炳朱舟谢雯佳
Owner SICHUAN UNIV
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