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A method for preserving the egg mass of Spodoptera frugiperda for multiplying Spodoptera spp.

A technology of Spodoptera frugiperda and Spodoptera, which is applied in the biological field of agriculture and forestry, can solve problems such as difficulty in accumulating egg sources and limitation of egg production, achieve simple operation process, increase parasitism rate, and avoid complex chemical and physical inactivation The effect of the operation

Active Publication Date: 2021-11-23
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, limited by the number of eggs laid by Spodoptera frugiperda and the rapid hatching of eggs, it is difficult to accumulate a certain scale and quantity of egg sources with consistent physiological indicators under indoor conditions for the expansion of Spodoptera frugiperda.

Method used

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  • A method for preserving the egg mass of Spodoptera frugiperda for multiplying Spodoptera spp.

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Effect test

Embodiment 1

[0034] A kind of fresh-keeping Spodoptera frugiperda ovum of the present invention is used for the method for multiplying Spodoptera spp., comprising the following steps:

[0035] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0036] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0037] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 1 cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing normal saline and dry it to remove possible pathogens, and dry it for storage;

[0038] (2) Fresh-keeping treatment process of Spodoptera frugip...

Embodiment 2

[0047] The method for the preservation of the fresh-keeping Spodoptera frugiperda ovum of the present invention for multiplying Spodoptera black egg bee comprises the following steps:

[0048] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0049] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0050] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 0.5cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing pure water and dry it to remove possible pathogens, and dry it for storage;

[0051] (2) Fresh-keeping treatment process of Spodop...

Embodiment 3

[0060] The method for the preservation of the fresh-keeping Spodoptera frugiperda ovum of the present invention for multiplying Spodoptera black egg bee comprises the following steps:

[0061] (1) Kraft paper is used as a carrier medium for adult fall armyworm eggs

[0062] a. Introduce the newly emerged adults of Spodoptera frugiperda into a 30×30×30cm insect cage, and put in cotton balls containing 10% honey water to supplement nutrition. After 2 days, hang a piece of kraft paper about 15×15cm in the center of the top of the cage It is used for trapping and laying eggs, as a carrier medium for trapping adults of Spodoptera frugiperda to lay eggs;

[0063] b. Replace the kraft paper every morning, check and cut off the piece of paper containing the egg mass at 0.8cm from the outer edge of the egg mass, rinse the egg mass with a syringe containing normal saline and dry it to remove possible pathogens, and dry it for storage;

[0064] (2) Fresh-keeping treatment process of Spo...

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Abstract

The invention discloses a method for preserving fresh Spodoptera frugiperda egg masses for multiplying and multiplying Spodoptera spp. Preservation under restricted conditions such as low temperature, while inhibiting the hatching of the eggs of Spodoptera frugiperda, ensured that the egg masses can be used for parasitism by the black egg wasp for a long time, and achieved the purpose of concentrating and multiplying the population of the black egg moth, which is convenient for timely The biological control effect of releasing parasitoid wasps in the field to control night greed in grassland. Its operation process is simple, safe and reliable, and provides a large number of parasitoid wasp sources for the convenient and quick use of the eggs of Spodoptera frugiperda to multiply the black egg wasp of Spodoptera frugiperda and the application of biological control technology for controlling Spodoptera frugiperda.

Description

technical field [0001] The invention belongs to the field of agriculture and forestry biotechnology, and relates to a method for preserving the eggs of host pests and multiplying their natural enemy parasitoids in a large amount indoors, in particular to a method for preserving fresh eggs of Spodoptera meadows and using them to multiply the natural enemies Spodoptera in large quantities The method of the black egg bee. Background technique [0002] Spodoptera frugiperda, commonly known as Fall armyworm (Fall armyworm), belongs to the Lepidoptera Noctuidae family Spodoptera genus, native to tropical and subtropical regions of America, is a major dangerous migratory that seriously threatens agricultural production Fly pests. The larvae of Spodoptera frugiperda have a large appetite and miscellaneous feeding habits, and can damage 353 species of host plants in 76 families. Since Spodoptera frugiperda invaded Jiangcheng County, Yunnan Province, my country in January 2019, it h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 金涛林玉英马光昌温海波刘俊彬
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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