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A kind of bacterial agent and application of Lactobacillus paracasei jlus66 with the function of improving memory impairment

A technology for improving memory and memory impairment, applied in lactobacillus, medical preparations containing active ingredients, applications, etc., can solve problems such as adverse reactions

Active Publication Date: 2022-05-13
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a kind of antibacterial agent and application for improving memory impairment, to overcome the defects of existing therapeutic drugs and adverse reactions of treatment methods

Method used

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  • A kind of bacterial agent and application of Lactobacillus paracasei jlus66 with the function of improving memory impairment
  • A kind of bacterial agent and application of Lactobacillus paracasei jlus66 with the function of improving memory impairment
  • A kind of bacterial agent and application of Lactobacillus paracasei jlus66 with the function of improving memory impairment

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preparation example Construction

[0032] In the present invention, the dosage form of the bacterial agent is preferably powder or water. The preparation method of the powder comprises the following steps: mixing the thalline of the strain with auxiliary materials to prepare the powder. The present invention does not have special restrictions on the preparation method of the auxiliary materials, and the auxiliary materials of food or medicine and health products well-known in the art can be used. When the bacterial agent is powder, the viable bacteria concentration of the Lactobacillus paracasei strain Jlus66 is preferably 10 7 ~10 8 CFU / g, more preferably 10 8 CFU / g. When the bacterial agent is an aqueous agent, the viable bacteria concentration of the Lactobacillus paracasei strain Jlus66 is preferably 10 8 ~10 9 CFU / mL, more preferably 10 9 CFU / mL. The water preparation is made by mixing the fermentation broth of the strain with a solvent to prepare the water preparation. The solvent is preferably st...

Embodiment 1

[0039] A method of expanding the culture of a strain of Lactobacillus paracasei Jlus66

[0040] The strain with preservation number of CGMCC No.22101 was streaked in MRS solid medium, cultured at 37°C for 48h, and a single colony with strong growth medium was selected and inoculated into 50mL MRS liquid medium, and cultivated at 37°C for 24h to obtain First-level activated seeds; then, the cells in the first-level seeds are centrifuged and concentrated, and the cells are collected and transferred to a shake flask containing 100 mL of MRS liquid medium, and cultivated under the same conditions to obtain the second-level seeds. Preparation of 100L fermentation medium (each liter of water contains the following weight components: peptone 10g, beef extract powder 5g, yeast extract 4g, glucose 20g, Tween-801.08g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tricitric acid Ammonium 2g, magnesium sulfate (MgSO 4 .7H 2 O) 0.2g, manganese sulfate (MnSO 4 .4H 2 O) 0.05g, pH...

Embodiment 2

[0042] 1. Source of experimental mice, grouping and processing methods

[0043] 1.1 C57BL / 6J male mice were selected, and adaptively fed for one week. During the adaptive feeding period, the mice were provided with commercial standard feed. After the adaptive feeding, the mice were randomly divided into three groups (n=10 / group). The high-fat group was fed a high-fat diet to construct an obese mouse model. Mice were housed in animal rooms with standard conditions: temperature 25±2°C, humidity 50%±5%, 12h light / dark cycle. All animal experiments followed the "Guidelines for the Protection and Use of Laboratory Animals" and "Administrative Measures for Laboratory Animals of Jilin University", and the animal protocol was provided by the Animal Ethics Committee of Jilin University.

[0044] 1.2 Grouping and processing methods

[0045] (1) Control group (Control): fed with standard feed, the calorie is 55kcal / cat / day;

[0046] (2) High-fat group (HFD): fed 50% high-fat feed, wit...

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Abstract

The invention provides a bacterial agent of Lactobacillus paracasei Jlus66 with the function of improving memory impairment and its application, belonging to the technical field of functional microorganisms. Experiments have shown that dietary supplementation of Lactobacillus paracasei strain Jlus66 can improve the hippocampal neuron damage caused by long-term high-fat diet, increase the expression of neurotrophic factor BDNF and inhibit the weakening of learning and memory, and improve memory impairment. It can be seen that the Lactobacillus paracasei strain Jlus66 has the potential to develop products for improving memory impairment, and it can be used as an auxiliary product for existing therapeutic drugs and treatment methods, and has great application value.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to a Lactobacillus paracasei Jlus66 bacterial agent with the function of improving memory impairment and its application. Background technique [0002] Hippocampus and central nervous system neurotrophic factors are crucial to human memory. Central nervous system neurotrophic factors play an important role in the survival and growth of neuronal cells. BDNF is the main neurotrophic factor in the mammalian brain. Studies have shown that long-term high-fat diet (HFD) can cause damage to hippocampal neurons in the brain and cause memory impairment. [0003] Different treatment methods and drugs should be used according to the severity of memory impairment. Although conventional drugs can play a certain therapeutic effect, they will produce adverse reactions such as liver damage, rash, and gastrointestinal reactions, and can only protect Nerve cells and ner...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/00A23L33/135C12R1/225
CPCA61K35/747A61P25/00A23L33/135A23V2002/00A23V2400/165A23V2200/322
Inventor 叶海青李圣楠张铁华赵昌辉沈雪
Owner JILIN UNIV
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