Lactobacillus paracasei Jlus66 microbial inoculum with function of improving memory disorder and application of lactobacillus paracasei Jlus66 microbial inoculum

A technology for improving memory and memory impairment, applied in Lactobacillus, medical preparations containing active ingredients, applications, etc., can solve problems such as adverse reactions

Active Publication Date: 2021-08-31
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a kind of antibacterial agent and application for improving memory impairment, to overcome the defects of existing therapeutic drugs and adverse reactions of treatment methods

Method used

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  • Lactobacillus paracasei Jlus66 microbial inoculum with function of improving memory disorder and application of lactobacillus paracasei Jlus66 microbial inoculum
  • Lactobacillus paracasei Jlus66 microbial inoculum with function of improving memory disorder and application of lactobacillus paracasei Jlus66 microbial inoculum
  • Lactobacillus paracasei Jlus66 microbial inoculum with function of improving memory disorder and application of lactobacillus paracasei Jlus66 microbial inoculum

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preparation example Construction

[0032] In the present invention, the dosage form of the bacterial agent is preferably powder or water. The preparation method of the powder comprises the following steps: mixing the bacterial cells of the bacterial strain with auxiliary materials to prepare the powder. The present invention has no special limitation on the preparation method of the excipients, and the excipients of food or medicine and health products well known in the art can be used. When the bacterial preparation was powder, the live bacterial concentration of the Lactobacillus paracasei bacterial strain Jlus66 was preferably 10 7 ~10 8 CFU / g, more preferably 10 8 CFU / g. When the bacterial agent is water agent, the live bacterial concentration of the Lactobacillus paracasei bacterial strain Jlus66 is preferably 10 8 ~10 9 CFU / mL, more preferably 10 9 CFU / mL. The water preparation is prepared by mixing the fermented liquid of the bacterial strain with a solvent. The solvent is preferably sterile wate...

Embodiment 1

[0039] A method for expanding culture of Lactobacillus paracasei bacterial strain Jlus66

[0040] Streak the strain with the preservation number CGMCC No.22101 in the MRS solid medium, culture it at 37°C for 48 hours, select a single colony that is robust in the medium and inoculate it into 50mL MRS liquid medium, and cultivate it at 37°C for 24h to obtain First-level activated seeds; then the bacteria in the first-level seeds were centrifuged and concentrated, and then the bacteria were collected and transferred to a shaker flask filled with 100mL MRS liquid medium, and cultivated under the same conditions to obtain second-level seeds. Prepare 100L fermentation medium (each liter of water contains the following components by weight: peptone 10g, beef extract powder 5g, yeast extract 4g, glucose 20g, Tween-801.08g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tricitric acid Ammonium 2g, magnesium sulfate (MgSO 4 .7H 2 O) 0.2g, manganese sulfate (MnSO 4 .4H 2 (2) 0...

Embodiment 2

[0042] 1. Source, grouping and treatment of experimental mice

[0043] 1.1 Select C57BL / 6J male mice, and first adapt to feed for one week. During the adaptive feeding, the mice are provided with commercial standard feed. After the adaptive feeding, the mice are randomly divided into three groups (n=10 / group). Among them, the high-fat group was fed a high-fat diet to construct an obese mouse model. The mice were kept in an animal room with standard conditions: temperature 25±2°C, humidity 50%±5%, 12h light / dark cycle. All animal experiments followed the Guidelines for the Protection and Use of Experimental Animals and the Measures for the Management of Laboratory Animals of Jilin University, and the animal protocol was provided by the Animal Ethics Committee of Jilin University.

[0044] 1.2 Grouping and processing methods

[0045] (1) Control group (Control): fed with standard feed, calorie 55kcal / bird / day;

[0046] (2) High-fat group (HFD): feed 50% high-fat feed, calorie...

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Abstract

The invention provides a lactobacillus paracasei Jlus66 microbial inoculum with a function of improving memory disorder and application, and belongs to the technical field of functional microorganisms. Experiments show that the dietary supplement lactobacillus paracasei strain Jlus66 can improve brain hippocampal neuron injury caused by long-term high-fat diet, increase neurotrophic factor BDNF expression and inhibit learning and memory ability weakening, and has the function of improving memory disorder. Therefore, the lactobacillus paracasei strain Jlus66 has the potential of developing the product for improving the memory disorder function, can be used as an auxiliary product of the existing treatment medicine and treatment method, and has great application value.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to a Lactobacillus paracasei Jlus66 bacterial agent with the function of improving memory impairment and its application. Background technique [0002] Hippocampus and central nervous system neurotrophic factors are crucial to human memory. Central nervous system neurotrophic factors play an important role in the survival and growth of neurons. BDNF is the main neurotrophic factor in the mammalian brain. Studies have shown that long-term high-fat diet (HFD) can cause damage to hippocampal neurons in the brain and cause memory impairment. In recent years, the number of patients with memory impairment has increased year by year. The main hazards are lost, suicide, and dementia. [0003] According to the severity of memory impairment, differentiated treatment should be carried out, and different treatment methods and drugs should be selected. Although conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P25/00A23L33/135C12R1/225
CPCA61K35/747A61P25/00A23L33/135A23V2002/00A23V2400/165A23V2200/322
Inventor 叶海青李圣楠张铁华赵昌辉沈雪
Owner JILIN UNIV
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