Application of protein and composition for identifying Tibetan pig in early pregnancy

An early pregnancy, composition technology, applied in biological testing, biological material analysis, material inspection products, etc., can solve problems such as no one has raised

Active Publication Date: 2021-11-16
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] It should be noted that no one has proposed corresponding identifi...

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  • Application of protein and composition for identifying Tibetan pig in early pregnancy
  • Application of protein and composition for identifying Tibetan pig in early pregnancy
  • Application of protein and composition for identifying Tibetan pig in early pregnancy

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Experimental program
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Embodiment Construction

[0039] The technical solutions of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but this does not constitute any limitation to the present invention.

[0040] (1) Obtaining serum of Tibetan pigs at different time points in early pregnancy

[0041] Select healthy female Tibetan pigs for the experiment, observe their estrus rules, and perform natural mating after they are naturally in estrus. For blood, after clotting the blood sample for 1 hour at room temperature, the serum was separated by centrifugation at 2000 g for 10 minutes. Serum samples were mixed with protease inhibitors (SigmaAldrich) and stored at −80 °C until further analysis. Sows were slaughtered on day 35 after mating and pregnancy was confirmed by the presence of embryos.

[0042] (2) Serum protein extraction and quality inspection

[0043] The total protein was extracted, quantified using the Bradford protein quantific...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to application of protein, and the protein is at least one of the following proteins: a talin1 protein with a sequence shown as an amino acid sequence NO.1; a Profilin protein, wherein the sequence of the Profilin protein is shown as an amino acid sequence NO.2; an HGF activator protein, wherein the sequence of the HGF activator protein is shown as an amino acid sequence NO.3; and a Carbonic anhydrase protein, wherein the sequence of the Carbonic anhydrase protein is shown as an amino acid sequence NO.4. The protein is used as a marker for Tibetan pig early pregnancy identification. Another purpose of the invention is to provide a protein marker composition for Tibetan pig early pregnancy identification.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the use of a protein and a composition for early identification of Tibetan pigs during pregnancy. Background technique [0002] Pig production is the core industry of animal husbandry, and the total global pork consumption has always ranked first in livestock products; pork has an irreplaceable position in the meat consumption of Chinese residents, and the efficiency of pork production has a profound impact on residents' lives. Pregnancy diagnosis of sows is a key link in the reproductive process of pigs, and the time and accuracy of the diagnosis greatly affect the reproductive performance of sows such as conception rate and litter lactation. Timely detection of empty pregnant sows is conducive to early arrangement of supplementary mating, shortening the empty pregnancy period of sows, shortening the farrowing interval, reducing the additional costs caused by ineffective...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/689G01N33/573G01N2333/47G01N2333/4704G01N2333/4706G01N2333/988
Inventor 辛海云孟繁明李宝红王塑天李剑豪胡斌
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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