Reagent and product for diagnosing diabetic nephropathy and application of reagent and product

A technology for diabetic nephropathy and reagents, applied in the field of diagnosis of diabetic nephropathy, can solve the problems of reducing microalbuminuria, poor correlation with diabetic nephropathy, affecting the judgment of nephropathy, etc., to achieve the effect of intervention and rapid and effective diagnosis

Inactive Publication Date: 2021-11-30
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in fact, microalbuminuria still has many deficiencies and limitations as a diagnostic marker, and the level of microalbuminuria in patients is affected by many other physiological and pathological factors in clinical practice, such as elevated body temperature, diet, body weight, etc. Wait
Secondly, the level of microalbuminuria is not stable. For example, the use of antihypertensive drugs may reduce the level of microalbuminuria and affect its judgment on renal lesions in patients with DN; and in the early stage of DN, the level of microalbuminuria and Diabetic nephropathy is less associated

Method used

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  • Reagent and product for diagnosing diabetic nephropathy and application of reagent and product
  • Reagent and product for diagnosing diabetic nephropathy and application of reagent and product
  • Reagent and product for diagnosing diabetic nephropathy and application of reagent and product

Examples

Experimental program
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Effect test

Embodiment 1

[0061] Example 1 Research on Biomarkers Related to Diabetic Nephropathy

[0062] The purpose of this study is to screen out biomarkers associated with diabetic nephropathy based on machine learning analysis, and to study its value in the diagnosis or prediction of diabetic nephropathy.

[0063] 1. Research methods

[0064] (1) The data and preprocessing methods used in the study

[0065] In the gene expression comprehensive database (GENE EXPRESSION OMNIBUS, GEO database), "diabetic nephropathy" was used as the keyword to search for public gene expression data related to diabetic nephropathy and its complete annotations. According to the clinical information recorded in the public gene expression data, Select clinical sample information from patients with diabetic nephropathy. The gene expression data of GSE30122 (normal:case=50:19) were downloaded from the GEO database (http: / / www.ncbi.nlm.nih.gov / geo / ), and the gene expression data from the GEO database (http: / / www.ncbi. ...

Embodiment 2

[0071] Example 2 Evaluation and analysis of the diagnostic efficacy of biomarkers TNFAIP6 and RUNX3

[0072] 1. Evaluation and analysis methods

[0073]Use the R package "pROC" to draw the receiver operating curve (ROC), and analyze the biomarkers TNFAIP6, RUNX3, and TNFAIP6+ that are significantly differentially expressed between the healthy control group and the diabetic nephropathy group obtained through the study in Example 1 The AUC value, sensitivity, and specificity of RUNX3 were used to judge the diagnostic efficacy of the indicators. Among them, when evaluating and analyzing the diagnostic efficacy of TNFAIP6 and RUNX3 for diabetic nephropathy, the expression level of the gene (log2 expression level) is used for evaluation and analysis, and the point corresponding to the largest Youden index is selected as its cutoff value, that is, the optimal division threshold passes When evaluating and analyzing the diagnostic efficacy of TNFAIP6+RUNX3 for diabetic nephropathy, t...

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Abstract

The invention discloses a reagent and product for diagnosing diabetic nephropathy and application of the reagent and the product and particularly relates to biomarkers TNFAIP6 and RUNX3. Expressions of the TNFAIP6 and the RUNX3 in patients with diabetic nephropathy are up-regulated, combination of the TNFAIP6 and the RUNX3 has relatively good diagnosis efficiency in diagnosis on the diabetic nephropathy, so that the TNFAIP6 and the RUNX3 can be applied to clinical diagnosis and treatment on the diabetic nephropathy as diagnosis and / or treatment targets.

Description

technical field [0001] The invention belongs to the field of genetic biological preparations, and in particular relates to reagents, products and applications for the diagnosis of diabetic nephropathy. Background technique [0002] Diabetic nephropathy is one of the most serious complications of diabetes. Diabetic nephropathy (DN) is the main microvascular complication of diabetes. DN mainly refers to diabetic glomerulosclerosis, a kidney disease mainly caused by vascular damage. Glomerular lesions. Early asymptomatic, blood pressure can be normal or high. Its incidence increases with the prolongation of the course of diabetes. In the early stage of diabetes, the kidney volume increases, the glomerular filtration rate increases, and it is in a hyperfiltration state. Interstitial proteinuria or microalbuminuria gradually appears. As the course of the disease prolongs, persistent proteinuria, edema, hypertension, glomerular Reduced filtration rate, and then renal insufficie...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68G16H50/20
CPCC12Q1/6883G01N33/6893G16H50/20C12Q2600/158G01N2800/042G01N2800/347G01N2333/47
Inventor 杨承刚李雨晨刘乐凯李丹
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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