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Method for purification of plasma proteins

A plasma protein and ligand technology, applied in the field of plasma protein purification, can solve the problems of easy loss of activity and unsatisfactory

Pending Publication Date: 2021-12-14
CYTIVA BIOPROCESS R&D AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, FVIII is prone to inactivation and known methods for purification are unsatisfactory in many respects

Method used

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  • Method for purification of plasma proteins
  • Method for purification of plasma proteins
  • Method for purification of plasma proteins

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Embodiment Construction

[0016] The present invention provides a magnetic bead capable of binding plasma proteins with high binding strength and capable of high binding capacity. This is achieved with plasma protein-binding magnetic beads comprising a porous matrix and one or more magnetic particles embedded in the matrix, wherein the matrix comprises a porous polymer, preferably agarose, and coupled to the porous polymer. Linked plasma protein binding ligand.

[0017] One advantage is that beads allow selective binding of large numbers of plasma proteins directly from crude material. Another advantage is that the beads have a favorable adsorption isotherm for plasma proteins, resulting in high yields of recovered plasma proteins.

[0018] The beads may suitably be sized such that a plurality of beads to be used in the methods disclosed hereinafter have a volume-weighted median diameter (d50, V) of 8-300 microns, such as 20-200, 20-100 microns or 20-80 microns . Beads of these sizes are easy to hol...

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Abstract

The present invention relates to a method for purification of plasma proteins. More closely, the invention relates to a method using magnetic beads for separation of different plasma proteins from a plasma fraction, such as a cryoprecipitate or cryosupernatant of plasma, or alternatively directly from cell culture of recombinant plasma proteins.

Description

technical field [0001] The invention relates to a method for purifying plasma proteins. More precisely, the present invention relates to a method for isolating different plasma proteins from plasma fractions, such as cryoprecipitate or cold supernatant of plasma, or directly from cell cultures of recombinant plasma proteins using magnetic beads. Background technique [0002] Blood contains different types of cells and molecules necessary for the function of a living body and is therefore collected for therapeutic purposes, for example for transfusion. However, different fractions can be isolated and prepared from blood, such as red blood cells or cell-free plasma, which enables more direct therapeutic treatment of medical conditions. Several proteins in plasma can also be further isolated and used for specific therapeutic treatments, such as albumin for restoring blood volume, immunoglobulins for immunodeficiency, and coagulation factors for coagulation disorders. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/755C07K14/76C07K16/06C12N9/74C07K14/47C07K1/18C07K1/22C07K1/14
CPCC07K14/76C07K14/755C12N9/644C07K14/47C07K16/065C07K1/22C07K14/745
Inventor M·哈尔M·安德尔M·贝格S·克里斯蒂安松
Owner CYTIVA BIOPROCESS R&D AB
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