Application of alpha-pinene in promoting growth of panax notoginseng and inducing resistance

A technology for inducing resistance and notoginseng, which is applied in the application field of α-pinene in promoting the growth of notoginseng and inducing resistance, can solve the problems of drug resistance of pathogenic bacteria, environmental hazards, high cost, etc., and achieve low acquisition cost, Source Rich Effects

Active Publication Date: 2022-01-07
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical treatment often uses some highly toxic chemical fumigants, which limit their use due to their harm to the environment, and also cause pathogenic bacteria to develop drug resistance; while physical treatment methods, such as steam sterilization, are also subject to high costs. limit

Method used

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  • Application of alpha-pinene in promoting growth of panax notoginseng and inducing resistance
  • Application of alpha-pinene in promoting growth of panax notoginseng and inducing resistance
  • Application of alpha-pinene in promoting growth of panax notoginseng and inducing resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: α-pinene promotes the germination experiment of Panax notoginseng seeds

[0053] The method of sand culture in a closed bottle is adopted: 100g of sterilized quartz sand is packed in a glass tissue culture bottle and then dried in an oven at 141° C. for 4 hours to sterilize. After cooling, add 18ml of sterilized ddH 2 O (double distilled water), stir well and plant 12 Panax notoginseng seeds. Take the tube cap of a 15ml centrifuge tube and place it on the quartz sand, add α-pinene with a concentration of 0, 5, 20, 50 and 100 μL / L (α-pinene volume / culture bottle volume) to the tube cap, and cap Put on the lid. After planting, they were uniformly placed in a constant temperature greenhouse at 25°C, and α-pinene was supplemented every 7 days. On the 50th day, start to investigate the situation of emergence, and then investigate once every 10d, and continuously investigate 5 times. The test results are shown in figure 1 middle.

[0054] The test results ...

Embodiment 2

[0055] Example 2: α-pinene induces Panax notoginseng plants to produce disease resistance

[0056] Use the fumigation method in a closed space: first select the potted plants of Panax notoginseng that grow well after transplanting, remove the weeds on the soil surface, and let the water soak in for 1 day, then cover the pot with a fresh-keeping bag so that the panax notoginseng is only on the ground. Part of it can be in contact with α-pinene to avoid the impact of α-pinene on the soil. Place the treated Panax notoginseng potted plants in transparent plastic sealed incubators, and then treat them with treatment concentrations of 0, 0.1, 1, 5, 20 and 50 μL / L (α-pinene volume / incubator volume) for 1d and 2d, respectively. And 3d induction time, supplemented with α-pinene once a day. After the Panax notoginseng seedlings were induced by α-pinene, the leaves were challenged to be inoculated with the bacteria cake of Alternaria panax SL17, and moved to a transparent plastic incuba...

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Abstract

The invention discloses application of alpha-pinene in promoting growth of panax notoginseng and inducing resistance. It is found that alpha-pinene fumigation can promote pre-germination of pseudo-ginseng seeds and induce pseudo-ginseng to generate disease resistance, especially resistance to pseudo-ginseng black spot. Alpha-pinene is a natural product, belongs to a plant source fumigant, has the effects of environmental protection, low toxicity and sustainable development, is rich in source and low in acquisition cost, and can be widely applied to panax notoginseng planting.

Description

[0001] This application is a divisional application of an invention patent application with an application date of May 18, 2021, an application number of 202110542601.5, and an invention title of "Application of α-pinene in Promoting Panax notoginseng Growth and Inducing Resistance". technical field [0002] The invention relates to the technical field of Panax notoginseng planting, in particular, the invention relates to the application of α-pinene in promoting the growth of Panax notoginseng and inducing resistance. Background technique [0003] Sanqi (Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, Dianqi, Shensanqi, Hansanqi, etc., is a perennial herbaceous Umbelliferae Araliaceae plant of the genus Ginseng, which has the functions of stopping bleeding, dissipating blood stasis, relieving pain and eliminating pain. It has good effects such as swollen and so on, and its tubers and flowers are mainly used as medicine (National Pharmacopoeia Commission, 2015; "Nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01P21/00A01G13/00
CPCA01N27/00A01G13/00
Inventor 朱书生叶辰黄惠川刘屹湘杨敏梅馨月邓维萍何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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