Detection method of rhodamine B

A detection method and technology to be detected, applied in measurement devices, preparation of test samples, instruments, etc., can solve problems such as the failure of low-concentration rhodamine B to meet the requirements, and achieve fast adsorption speed, good reproducibility and stability the effect of low detection limit

Pending Publication Date: 2022-02-18
南宁兴科净医疗科技有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a detection method for rhodamine B, aiming at solving the problem that the detection of low-concentration rhodamine B in complex food matri

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  • Detection method of rhodamine B
  • Detection method of rhodamine B
  • Detection method of rhodamine B

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Embodiment Construction

[0036] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein like or similar reference numerals designate like or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0037] see Figure 1 to Figure 4 , the invention provides a kind of detection method of rhodamine B, comprises the steps:

[0038] S1: Preparation of 3D graphene / Fe 3 o 4 ;

[0039] S2: Process the tested sample to obtain a standard working sample;

[0040] S3: Using 3D Graphene / Fe 3 o 4 Perform testing on standard working samples.

[0041] Among them, the preparation of 3D graphene / Fe 3 o 4 The steps include:

[0042] Prepare graphene oxide;

[0043] Using graphene oxide to prepare 3D graphene;

[0044] Fabrication of 3D gra...

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Abstract

The invention discloses a detection method of rhodamine B. A 3D graphene/Fe3O4 magnetic composite material is prepared through a coprecipitation method, wherein magnetic solid-phase extraction is constructed to be combined with a fluorospectro photometer to detect rhodamine B in food. Strong pi-pi interaction and electrostatic attraction exist between the graphene and the rhodamine B, and the strong adsorption effect of the graphene on the rhodamine B is achieved through mutual attraction between the graphene and the rhodamine B; and the Fe3O4 is combined with graphene, so that the adsorption separation process is simple and rapid. The adsorption isotherm accords with a Langmuir isothermal adsorption model, the adsorption capacity is 22.09 mg/g, the adsorption kinetic data fitting accords with a quasi-secondary kinetic equation, and the balance can be reached after 10 minutes of adsorption. The established detection method is simple, rapid, high in sensitivity and wide in linear range.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting rhodamine B. Background technique [0002] Rhodamine B (RhB) is a well-known water-soluble dye with strong fluorescent properties and long-lasting dyeing ability. It is commonly used in laboratory cell stains and industrial dyes, such as papermaking, printing and dyeing, cosmetics and printing industries. Rhodamine B can irritate the skin, eyes and respiratory tract; in mammals, Rhodamine B can combine with bovine serum albumin (BSA), change the microenvironment of tryptophan and the conformation and molecular energy level of BSA, and prevent protein synthesis , has potential reproductive and developmental toxicity, carcinogenicity and neurotoxicity. Rhodamine B is illegally added to chili powder and other foods in order to maintain or increase the bright color of the food, which will cause great potential harm to the health of consumers. Therefore...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
CPCG01N21/6402G01N1/28
Inventor 严军唐海林陈林朱伟伟
Owner 南宁兴科净医疗科技有限公司
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