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A kind of green foxtail alga functional feed and preparation method thereof

A technology of foxtail algae and functional feed, which is applied to animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of secondary pollution, reduce feed cost, avoid secondary pollution, and reduce sewage The effect of processing fees

Active Publication Date: 2022-07-08
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the above problems, the object of the present invention is to provide a method for preparing a viridiflorum functional feed, and the viridiflorium functional feed produced by the method improves the palatability of the viridiflorium feed, contributes to Maintain the balance of animal intestinal flora, promote the production of beneficial metabolites, and at the same time solve the problem of secondary pollution caused by the treatment of waste water by P. viridis, and realize the mutual unity of environmental protection and resource development and utilization

Method used

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  • A kind of green foxtail alga functional feed and preparation method thereof
  • A kind of green foxtail alga functional feed and preparation method thereof
  • A kind of green foxtail alga functional feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Probiotic properties of Lactobacillus plantarum BW2013

[0067] 1. In vitro simulated gastrointestinal digestion experiment

[0068] 1) Preparation of artificial saliva

[0069] 6.2g NaCl, 2.2g KCl, 0.22g CaCl 2 , 1.2g NaHCO 3 Dissolve in 1L deionized water and autoclave. Cool to about 20°C, add 3.0g / L α-amylase, adjust the pH to 6.9, and filter and sterilize with a 0.22-micron microporous membrane.

[0070] 2) Preparation of artificial gastric juice

[0071] 3.0g NaCl, 1.1g KCl, 0.15g CaCl 2 , 0.60g NaHCO 3 It was prepared into 1L solution and sterilized by high pressure steam; cooled to about 20℃, added 3.0g / L of pepsin obtained from porcine gastric mucosa, adjusted the pH to 2.0 with 10N HCl, and sterilized by filtration with a 0.22-micron microporous membrane.

[0072] 3) Preparation of artificial small intestinal fluid

[0073] 5.0g NaCl, 0.60g KCl, 0.30g CaCl 2 , 0.60g NaHCO 3 Dissolve in 1L deionized water and autoclave. Cool to about 20°C, ...

Embodiment 2

[0081] Example 2 The probiotic effect of Lactobacillus plantarum BW2013

[0082] 1. Preparation of DSS: prepare dextran sodium sulfate solution with a mass fraction of 2.5%, and the solvent is phosphate buffered saline.

[0083] 2. Preparation of Lactobacillus plantarum BW2013 live bacterial suspension: Inoculate Lactobacillus plantarum BW2013 in 500ml of MRS liquid medium according to the inoculum amount of 10% (w / w), and culture at 37°C for 18h, at 8000r / min The cells were collected by centrifugation for 15 min; the cell pellets were resuspended in phosphate buffered saline and prepared into 2.34×10 8 CFU / ml low-dose bacterial suspension L (DL group), 2.34×10 9 CFU / ml medium-dose bacterial suspension M (DM group), 2.34×10 10 CFU / ml high-dose bacterial suspension H (DH group), NC as normal control group (sterile PBS), D group as model group successfully established with DSS, PC as mesalazine positive control group.

[0084] 3. Modeling: 72 BALB / c mice were randomly divided...

Embodiment 3

[0118] Example 3: Preparation of the functional feed of Pseudomonas viridis

[0119] 1. Feed preparation steps

[0120] 1) Raw material cutting

[0121] Clean the harvested fresh pink Myriophyllum aquaticum to ensure that the samples are free of mold and deterioration. The cleaned samples are dried to reduce their water content to 55-65%, and then cut into 1±0.5cm pieces. small paragraphs.

[0122] 2) Inoculation mix

[0123] Will 1x10 9 The bacterial solution of Lactobacillus plantarum BW2013 with a concentration of CFU / mL was evenly sprayed on the dried and cut Pseudomonas viridis according to the inoculum amount of 10% (w / w), and then 6% (w / w) of sucrose solid was added, Mix well to make a mixture.

[0124] 3) bagging and sealing

[0125] Pack the mixture into a sterile plastic bag and seal it with a small vacuum sealer to remove the air.

[0126] Pack the 500g mixed material into a sterile plastic bag, exhaust the air with a small vacuum, and seal it. The amount of...

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Abstract

The present invention provides a method for preparing a functional feed of Chlorella viridis, which comprises the following steps: 1) cutting raw materials; 2) inoculating and mixing materials; 3) bagging and sealing; Live bacteria mix. The functional feed prepared by the invention adopts Lactobacillus plantarum to ferment, increases the protein content, fat content and ash content in the chlorophyll algae, and reduces the cellulose content in the fenugreek algae. The feed not only improves the nutritive value and palatability of the feed of Chlorella chinensis, and is convenient for storage and transportation; but also improves the structure of animal intestinal flora and promotes the production of beneficial metabolites, which can replace 10%-20% of conventional feeds At the same time, it also solves the problem of secondary pollution easily caused by the treatment of aquaculture wastewater, and realizes the mutual unity of environmental protection and resource development and utilization.

Description

technical field [0001] The invention belongs to the technical field of feed processing, and relates to a functional feed of Pseudomonas viridis and a preparation method thereof. Background technique [0002] In recent years, the global food crisis has intensified, and international food prices have risen sharply. my country's feed grain accounts for 1 / 3 of the total grain output, and 40.0% of the arable land is used for feed production. With the rapid development of my country's animal husbandry industry, protein and energy feed resources are in short supply, and the cost of breeding is too high. The shortage of feed resources has always affected the sustainable development of animal husbandry and has become a major challenge for my country's animal husbandry. The development and utilization of grain and feed is an urgent problem to be solved in the development of the current aquaculture industry. [0003] At the same time, with the vigorous development of my country's aquac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/30A23K10/12A23K20/163
CPCA23K10/30A23K10/12A23K20/163A23V2400/169Y02P60/87
Inventor 李祖明
Owner BEIJING UNION UNIVERSITY
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