Feces lactoferrin detection kit and preparation method thereof

Abstract

The invention provides an excrement lactoferrin detection kit and a preparation method thereof. The excrement lactoferrin detection kit comprises a PVC bottom plate, and a sample pad, a colloidal gold label pad, a nitrocellulose membrane and absorbent paper are sequentially arranged on the PVC bottom plate from left to right in a lap joint mode; a detection line T, a control line R and a quality control line C are sequentially distributed in the nitrocellulose membrane from left to right, and a detection region T, a control region T and a quality control region C are respectively formed on the nitrocellulose membrane at the corresponding positions of the detection line T, the control line R and the quality control line C; the right side end part of the sample pad is lapped on the upper side of the left side end part of the colloidal gold label pad, the right side end part of the colloidal gold label pad is lapped on the upper side of the left side end part of the nitrocellulose membrane, and the left side end part of the absorbent paper is lapped on the upper side of the right side end part of the nitrocellulose membrane; the whole operation is simple, the performance is reliable, the faeces lactoferrin is rapidly and accurately qualitatively detected, whether the sample to be detected contains the lactoferrin or not is visually judged, the cost is low, and the method is suitable for clinical rapid detection.

Description

【Technical field】 [0001] The invention relates to medical detection technology, in particular to a fecal lactoferrin detection kit and a preparation method thereof. 【Background technique】 [0002] Diarrhea is a very common clinical symptom, but there are many difficulties and problems in the diagnosis and treatment of diarrhea. The etiology of diarrhea is diverse, and if a simple test could determine whether diarrhea is inflammatory in nature, then many invasive diagnostic methods would not be necessary. [0003] Leukocytes in feces represent inflammation in the intestinal tract, so the detection of leukocytes in feces is particularly important, but the detection method is strict and difficult to operate clinically. Lactoferrin (LF) is an iron-binding glycoprotein discovered in recent years. It is secreted by most mucous membranes and is the main component of secondary granules of polymorphonuclear neutrophils. It is mainly distributed and expressed in neutrophils , epithe...

AI Technical Summary

Problems solved by technology

[0003] Leukocytes in feces represent inflammation in the intestinal tract, so the detection of leukocytes in feces is particularly important, but the detection method is strict and difficult to operate clinically

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