Inducer for attachment metamorphosis of urechis unicinctus larvae as well as preparation method and application of inducer

A single-ringed stinger, attachment metamorphosis technology, applied in the application, additional food elements, fish farming and other directions, can solve the problems of the single-ringed sting larvae's low metamorphosis rate and the like, and achieve the effect of improving the attachment metamorphosis rate

Pending Publication Date: 2022-05-03
中国海洋大学三亚海洋研究院
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AI Technical Summary

Problems solved by technology

However, the rate of metamorphosis of the larvae of E. monocircleum was low when feeding the bait

Method used

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  • Inducer for attachment metamorphosis of urechis unicinctus larvae as well as preparation method and application of inducer
  • Inducer for attachment metamorphosis of urechis unicinctus larvae as well as preparation method and application of inducer
  • Inducer for attachment metamorphosis of urechis unicinctus larvae as well as preparation method and application of inducer

Examples

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preparation example Construction

[0028] The present invention also provides a preparation method of the above-mentioned inducer, comprising the following steps: mixing the FILa6 mature peptide with double distilled water to obtain a storage solution, and then mixing with seawater to obtain the inducer.

[0029] In the present invention, the concentration of the FILa6 mature peptide in the storage solution is preferably 2.5-15 mg / mL, more preferably 5 mg / mL.

[0030] The present invention also provides the application of the above-mentioned inducer in inducing attachment and metamorphosis of the larvae of C. monocircleum.

[0031] In the present invention, the method of induction preferably includes adding the larvae of Echinocyst monocircleum to the inducer for induction for 24-48 hours.

[0032] In the present invention, the induction temperature is preferably 19-20°C, more preferably 19.5°C.

[0033] The present invention has no special limitation on the sources of raw materials not mentioned, and commerci...

Embodiment 1

[0036] Synthesis of FILa6 mature peptide

[0037] The FILa6 mature peptide sequence (SMPQYDDFIL) was sent to Shanghai Sangon Biotechnology Co., Ltd., and the C-terminus of the mature peptide was amidated to synthesize an active FILa6 mature peptide. The synthetic purity of the FILa6 mature peptide was greater than 98%.

Embodiment 2

[0039] The FILa6 mature peptide obtained in Example 1 was mixed with 7.36 μL of double-distilled water to obtain a stock solution with a concentration of 5 mg / mL, and then mixed with 3 mL of seawater with a salinity of 29 to obtain an inducer with a concentration of 10 μM of the FILa6 mature peptide.

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Abstract

The invention provides an inducer for attachment metamorphosis of urechis unicinctus larvae as well as a preparation method and application of the inducer, and belongs to the technical field of urechis unicinctus breeding. The inducer comprises FILa6 mature peptide, double distilled water and seawater, and the concentration of the FILa6 mature peptide is specifically limited to be 5-30 [mu] M. According to the inducer containing the FILa6 mature peptide, the attachment metamorphosis rate of urechis unicinctus larvae can be remarkably increased. Experimental results show that the adhesion and metamorphosis rate is 53.3% when the inducer induces for 24 h, and the adhesion and metamorphosis rate is as high as 93.3% when the inducer induces for 48 h and is obviously higher than that of a control group.

Description

technical field [0001] The invention belongs to the technical field of Echinacea monoringensis breeding, and in particular relates to an inducer for attachment metamorphosis of Echinacea monoringensis larvae, a preparation method and application thereof. Background technique [0002] The phylum Echiura is a class of marine benthic invertebrates. Urechisunicinctus (Urechisunicinctus), commonly known as sea intestine, is a kind of tubeless weedid (Xenopneusta) distributed in U-shaped caves in intertidal sediments along the coasts of China, Korea, Japan and Russia (Li Nuo et al. , 1995; Chen Zongtao, 2005), is also a new type of marine economic invertebrates. In recent years, commodity prices have doubled, approaching or even exceeding the price of fresh sea cucumbers. At present, C. monoringensis on the market mainly comes from coastal harvesting, but due to the brutality and destructiveness of its fishing, the natural population is seriously threatened. Therefore, it is immi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/147A23K20/20A01K61/40
CPCA23K50/80A23K20/147A23K20/20A01K61/40
Inventor 张龙张志峰杨智陈宗涛张正睿
Owner 中国海洋大学三亚海洋研究院
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