Inducer for attachment metamorphosis of urechis unicinctus larvae as well as preparation method and application of inducer
A single-ringed stinger, attachment metamorphosis technology, applied in the application, additional food elements, fish farming and other directions, can solve the problems of the single-ringed sting larvae's low metamorphosis rate and the like, and achieve the effect of improving the attachment metamorphosis rate
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[0028] The present invention also provides a preparation method of the above-mentioned inducer, comprising the following steps: mixing the FILa6 mature peptide with double distilled water to obtain a storage solution, and then mixing with seawater to obtain the inducer.
[0029] In the present invention, the concentration of the FILa6 mature peptide in the storage solution is preferably 2.5-15 mg / mL, more preferably 5 mg / mL.
[0030] The present invention also provides the application of the above-mentioned inducer in inducing attachment and metamorphosis of the larvae of C. monocircleum.
[0031] In the present invention, the method of induction preferably includes adding the larvae of Echinocyst monocircleum to the inducer for induction for 24-48 hours.
[0032] In the present invention, the induction temperature is preferably 19-20°C, more preferably 19.5°C.
[0033] The present invention has no special limitation on the sources of raw materials not mentioned, and commerci...
Embodiment 1
[0036] Synthesis of FILa6 mature peptide
[0037] The FILa6 mature peptide sequence (SMPQYDDFIL) was sent to Shanghai Sangon Biotechnology Co., Ltd., and the C-terminus of the mature peptide was amidated to synthesize an active FILa6 mature peptide. The synthetic purity of the FILa6 mature peptide was greater than 98%.
Embodiment 2
[0039] The FILa6 mature peptide obtained in Example 1 was mixed with 7.36 μL of double-distilled water to obtain a stock solution with a concentration of 5 mg / mL, and then mixed with 3 mL of seawater with a salinity of 29 to obtain an inducer with a concentration of 10 μM of the FILa6 mature peptide.
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