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A method for improving coral larva attachment metamorphosis

A technology for attaching metamorphosis and larvae, applied in climate change adaptation, application, fish farming, etc., can solve the problems of global coral reef ecosystem degradation, coral reef biome distribution, structure and function impact, etc., to shorten the time of larvae metamorphosis, The effect of improving the adhesion metamorphosis rate and easy operation

Active Publication Date: 2020-08-04
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the interference of factors such as human activities and changes in the natural environment in the past century, the distribution, structure and function of the coral reef biome are gradually being affected, and the global coral reef ecosystem has been seriously degraded.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] On September 13, 2018, at the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the Pocillopora larvae were collected from the Luhuitou waters, and placed in a filter tank. In the 6-well plate of seawater, the seawater is filtered with a 0.45 μm filter membrane. Collect shell-shaped coralline algae from Luhuitou, remove the miscellaneous algae and wash it with sea water, and make it into a size of about 1cm 2 A block-shaped algal body, one piece of prepared shell-shaped coralline algae is placed in each orifice plate. Under the conditions of a culture temperature of 26-27° C. and a salinity of 33 ppt, the culture was statically cultivated for 2 days without aeration or water change during the cultivation process. After testing, adopting the method of the present invention, the attachment rate of 4h Pocillopora larvae is as high as 73%, and the metamorphosis rate of attached coral larvae tend...

Embodiment 2

[0016] On September 13, 2018, at the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the Pocillopora larvae were collected from the Luhuitou waters, and placed in a filter tank. In the 6-well plate of seawater, the seawater is filtered with a 0.45 μm filter membrane. Collect shell-shaped coralline algae from Luhuitou, remove the miscellaneous algae and wash it with sea water, and make it into a size of about 2cm 2 A block-shaped algal body, one piece of prepared shell-shaped coralline algae is placed in each orifice plate. Under the conditions of a culture temperature of 26-27° C. and a salinity of 33 ppt, the culture was statically cultivated for 2 days without aeration or water change during the cultivation process. After testing, adopting the method of the present invention, the attachment rate of 4h Pocillopora larvae is as high as 75%, and the metamorphosis rate of attached coral larvae tend...

Embodiment 3

[0018] On September 15, 2018, at the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the Pocillopora larvae were collected from the Luhuitou waters and placed in a filter tank. In the 6-well plate of seawater, the seawater is filtered with a 0.45 μm filter membrane. The cylindrical algae cultivated in the laboratory are made into a size of about 1cm 2 The box-shaped algal body of each hole plate is put into 1 piece of prepared cylindrical cactus algae. Under the conditions of a culture temperature of 26-27° C. and a salinity of 33 ppt, the culture was statically cultivated for 2 days without aeration or water change during the cultivation process. After testing, adopting the method of the present invention, the attachment rate of Pocillopora larvae in 12 hours is 76%, and the metamorphosis rate of attached coral larvae tends to be 100%.

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Abstract

The invention discloses a method for improving attachment metamorphosis of coral larvae. The method comprises the following steps of transferring the coral larvae into a vessel filled with filtered seawater, adding clean calcified algae into the vessel, culturing the coral larvae for 2 days under the conditions that the culture temperature is 26-27 DEG C and the salinity reaches 33 ppt, and observing the attachment metamorphosis condition of the larvae without water replacement and inflation in the culturing process. By using the method, the attachment rate of the coral larvae is increased from 1% or below to 70% or above within 4-12 hours, and the metamorphosis rate of the attached coral larvae approaches 100%. The method has the advantages of being easy and convenient for operation, obvious in induction effect and capable of obviously increasing the attachment metamorphosis rate of the coral larvae and providing a technical guarantee for large-scale cultivation of coral seedlings.

Description

technical field [0001] The invention relates to the technical field of coral breeding, in particular to a method for improving the attachment and metamorphosis of coral larvae. Background technique [0002] Coral reefs are one of the ecosystems with the highest primary production in the world and have important ecological, economic and social values. However, due to the interference of factors such as human activities and changes in the natural environment in the past century, the distribution, structure and function of the coral reef biome are gradually being affected, and the global coral reef ecosystem has been seriously degraded. As an important class of calcifying organisms, reef-building corals are the main builders of coral reefs. The study of coral artificial propagation technology is of great significance to the restoration and reconstruction of coral reef ecosystems. In the production of coral seedlings, since the larvae are sensitive to changes in external envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 杨芳芳龙丽娟莫嘉豪韦章良
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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