Liquid phase chip method hybridization buffer solution, preparation method and liquid phase chip detection method
A liquid phase chip, hybridization buffer technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as difficulty in use, crystallization, and insoluble SDS, to reduce background signals, improve sensitivity, and composition simple effect
Active Publication Date: 2022-07-08
JIHUA LAB
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[0006] This formula has the following disadvantages: 1. The SDS added in the preparation process is extremely difficult to dissolve in the buffer
2. The total ion concentration of the hybridization buffer is high, and crystallization is easy to occur in an environment with a temperature lower than 10°C, which makes it difficult to use
[0007] At present, there are no related reports on the screening and optimization of hybridization buffer for liquid-phase chip method, and the sensitivity of hybridization detection needs to be further improved
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[0046] In order to make the objectives, technical solutions and effects of the present invention clearer and clearer, the present invention will be described in further detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0047] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.
[0048] Most of the buffers screened and optimized in the existing literature are PCR buffers, that is, in the above process of nucleic acid detection using the liquid chip method, the PCR buffer is used in the process (2) PCR amplification reaction, and the present invention is aimed at Process (3) Hybridization buffer used in the hybridization reaction of probe microspheres and PCR products.
[0049] It should be noted that the buffer used in the hybridization reaction is completely differen...
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The invention belongs to the field of liquid-phase chips, discloses a liquid-phase chip method hybridization buffer solution, particularly relates to a hybridization buffer solution suitable for liquid-phase chip method multiple nucleic acid detection, and provides a preparation method and a method for liquid-phase chip detection by using the hybridization buffer solution. After improvement on the requirement that crystals are not separated out at low temperature, the concentration of TMAC in a traditional liquid-phase chip method hybridization buffer solution is adjusted, ammonium chloride which is not used in the liquid-phase chip method hybridization buffer solution is added, and it is accidentally found that the liquid-phase chip method hybridization buffer solution can reduce background signals in detection of multiple PCR samples; the hybridization reaction sensitivity is improved.
Description
technical field [0001] The invention relates to a liquid-phase chip method hybridization buffer, a preparation method and a liquid-phase chip detection method, and belongs to the field of liquid-phase chips. Background technique [0002] The liquid-phase chip is also called suspension array or flow fluorescence technology. Its basic detection principle is to use suspended microspheres as the liquid-phase reaction carrier and flow cytometry as the analysis method. Reporter molecule fluorescence to achieve the purpose of qualitative and quantitative detection. Just by incorporating different ratios of red and orange fluorescent dyes, the microspheres can be divided into more than 100 different colors to form a coded microsphere array, which enables one-time high-throughput and high-density analysis of multiple indicators. Therefore, liquid chip technology has great application potential in the large-scale detection of biological macromolecules such as nucleic acids and protei...
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IPC IPC(8): C12Q1/6837
CPCC12Q1/6837
Inventor 顾桐旭褚春旭钟嘉俊白鹏利王哲
Owner JIHUA LAB