Application of biomarker in diagnosis and prognosis of HBV (Hepatitis B Virus) related liver cancer
A liver cancer prognosis and liver cancer technology, which is applied in the field of medical biological detection, can solve the problems that the lesion location is not necessarily accurate, is not suitable for early diagnosis, and is not applicable, and achieves a non-invasive method, improved sensitivity and specificity, and a simple and convenient method. Effect
Active Publication Date: 2022-08-02
南昌大学第一附属医院
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Problems solved by technology
The acquisition of tissue samples is an invasive process, and the location of the lesion may not be accurate, so it is not suitable for early diagnosis or screening of high-risk groups
Method used
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[0030] The present invention will be further described in detail below with reference to specific embodiments. However, it should not be construed that the scope of the above-mentioned subject matter of the present invention is limited to the following embodiments, and all technologies realized based on the content of the present invention belong to the scope of the present invention. Unless otherwise specified, the technical means and operation methods used in the following examples are conventional means and methods well known to those skilled in the art, and the raw materials used are all commercially available commodities.
[0031] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0032] 1. Serum RNA extraction
[0033] Follow the kit instructions (miRNeasy Serum / Plasma Kit, Qiagen 217184).
[0034] 1. Prepare 400 µl serum and thaw frozen samples;
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The invention provides an application of a reagent for detecting the expression level of lncRNA in preparation of a product for diagnosing HBV-related liver cancer or predicting prognosis of HBV-related liver cancer, the lncRNA is AC005332.5, ELF3-AS1 and LINC00665, the marker combination has high specificity and sensitivity, can be used as an effective diagnosis marker for HBV-related liver cancer, and can be used for preparing a product for diagnosing HBV-related liver cancer or predicting prognosis of HBV-related liver cancer. And the expression level of the gene is related to the staging, grading and overall survival rate of the HBV-related liver cancer, and the gene can be applied to prognosis of the HBV-related liver cancer.
Description
technical field [0001] The invention relates to the technical field of medical biological detection, in particular to the application of serum markers in the diagnosis and prognosis judgment of HBV-related liver cancer. Background technique [0002] Hepatocellular carcinoma (HCC, hereinafter referred to as liver cancer) is one of the most common clinical malignant tumors, and its mortality rate has jumped to the third place among malignant tumors in recent years, seriously threatening human life and health. Hepatitis B virus (HBV) is considered to be a key risk factor for the progression of liver cancer, and even a major cause. Despite advances in treatments such as surgery, transplantation, and radiofrequency ablation, patients with HBV-related hepatocellular carcinoma still have a low overall survival rate due to the high tumor invasiveness and recurrence rate. And for most patients with HBV-related liver cancer, at the time of diagnosis, it has progressed to the middle a...
Claims
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Login to View More IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/178C12Q2600/158C12Q2600/118
Inventor晏少颖龚彬彬
Owner南昌大学第一附属医院