Process of extracting glasswort oil from glasswort plant

Abstract

The invention is a method of extracting active substance-sea Erigeron acris seed oil from natural plant sea Erigeron acris, adding water to the dried and crushed or pulped whole plants or seeds of sea Erigeron acris to blend, heat and cook, then cooling to 40 deg.C-60 deg.C, adding enzyme preparation to regulate the pH value to 2-10 so as to make enzymolysis for 1-20 hours, heating and filtering deposit, taking enzymolysis solution to extract mixed oil of sea Erigeron acris seeds by organic solvent; or using linolic acid isomerizing enzyme to convert the linolic acid in the mixed oil to conjugated linolic acid. The mixed oil extraction ratio is up to 29%-35%, and the ratio of linolic acid to sea Erigeron acris oil >=74%.

Description

Technical field:

[0001] The invention relates to a method for extracting natural active substances from natural plants, in particular to a method for extracting salicornia oil from salicornia plants. Background technique:

[0002] Salicornia bigclvii Torr is native to coastal and inland salt marshes in temperate and subtropical regions of North America. It is a leafless, succulent annual salt marsh plant with high seed yield. The whole body of Salmonella is a treasure, and its tender stems and leaves can be eaten as vegetables, and its seed oil content is higher than 35%. The linoleic acid in samphire mixed oil contains 74% and 2.6% linolenic acid, among which linoleic acid is a fatty acid that is necessary for the healthy growth of the human body but cannot be synthesized by the human body itself. It has the functions of nourishing brain cells and regulating the function of autonomic nerves It participates in the contraction of human smooth muscle, the activity of enzymes ...

Examples

Embodiment 1

[0012] Take the dried Salicornia plant, crush it, add water 3 times the weight of the raw material, heat and cook for 1 hour after fully stirring, and when it is allowed to cool to 50°C, add 0.5% of the original weight of protease to adjust the pH value to 5.0, and then keep the temperature at Enzymatic hydrolysis at 55°C for 5 hours. After enzymolysis, heat it at 100°C and filter for 30 minutes, take the enzymolysis solution and add petroleum ether for extraction, and recover petroleum ether to obtain samphire oil.

Embodiment 2

[0014] Take 2 kg of dry samphire seeds, add 5 kg of water, heat and cook for 2 hours after fully stirring, add 5 grams of protease, 5 grams of cellulase, and 5 grams of pectinase when cooled to 60 ° C, adjust the pH value to 5.5, and then keep Enzymolysis was carried out at 60°C for 6 hours. After enzymolysis, heat it at 90°C to filter the precipitate for 45 minutes, take the enzymolysis solution and add methanol for extraction, and recover methanol to obtain Salicornia oil.

Embodiment 3

[0016] Take 1 kg of salicornia oil, add 10 grams of linoleic acid isomerase (Lactobacillus Yeuferi PYR8 (Atcc 55739)), adjust the pH value to 7, and cultivate it for 42 hours at 35-37°C, and the salicornia oil can be extracted The linoleic acid is converted into conjugated linoleic acid.

Technical field:

[0001] The invention relates to a method for extracting natural active substances from natural plants, in particular to a method for extracting salicornia oil from salicornia plants. Background technique:

[0002] Salicornia bigclvii Torr is native to coastal and inland salt marshes in temperate and subtropical regions of North America. It is a leafless, succulent annual salt marsh plant with high seed yield. The whole body of Salmonella is a treasure, and its tender stems and leaves can be eaten as vegetables, and its seed oil content is higher than 35%. The linoleic acid in samphire mixed oil contains 74% and 2.6% linolenic acid, among which linoleic acid is a fatty acid that is necessary for the healthy growth of the human body but cannot be synthesized by the human body itself. It has the functions of nourishing brain cells and regulating the function of autonomic nerves It participates in the contraction of human smooth muscle, the activity of enzymes ...

Examples

Embodiment 1

[0012] Take the dried Salicornia plant, crush it, add water 3 times the weight of the raw material, heat and cook for 1 hour after fully stirring, and when it is allowed to cool to 50°C, add 0.5% of the original weight of protease to adjust the pH value to 5.0, and then keep the temperature at Enzymatic hydrolysis at 55°C for 5 hours. After enzymolysis, heat it at 100°C and filter for 30 minutes, take the enzymolysis solution and add petroleum ether for extraction, and recover petroleum ether to obtain samphire oil.

Embodiment 2

[0014] Take 2 kg of dry samphire seeds, add 5 kg of water, heat and cook for 2 hours after fully stirring, add 5 grams of protease, 5 grams of cellulase, and 5 grams of pectinase when cooled to 60 ° C, adjust the pH value to 5.5, and then keep Enzymolysis was carried out at 60°C for 6 hours. After enzymolysis, heat it at 90°C to filter the precipitate for 45 minutes, take the enzymolysis solution and add methanol for extraction, and recover methanol to obtain Salicornia oil.

Embodiment 3

[0016] Take 1 kg of salicornia oil, add 10 grams of linoleic acid isomerase (Lactobacillus Yeuferi PYR8 (Atcc 55739)), adjust the pH value to 7, and cultivate it for 42 hours at 35-37°C, and the salicornia oil can be extracted The linoleic acid is converted into conjugated linoleic acid.

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